Yoshida T, Takeuchi M
Institute for Fermentation, Osaka, Japan.
J Neurosci Res. 1993 Jun 1;35(2):129-37. doi: 10.1002/jnr.490350203.
An immortalized clonal cell line (AP-16) has been established from glial cultures obtained from neonatal mouse cerebra by multipassages under serum-free conditions. Immunofluorescent experiments showed that AP-16 cells expressed a marker for glial progenitors (A2B5) and did not express markers for oligodendrocytes (galactocerebroside) or mature astrocytes (glial fibrillary acidic protein: GFAP). Treatment with transforming growth factor-beta 1 (TGF-beta 1) or fetal calf serum (FCS) for 2 days induced AP-16 cells to differentiate into A2B5-negative, GFAP-positive, phenotypically mature astrocytes. AP-16 cells depended on epidermal growth factor for survival, and their growth was inhibited by FCS. These results indicate that AP-16 cells retained the properties of astrocyte progenitors. An enzyme-linked immunosorbent assay showed that AP-16 cells synthesized fibronectin and laminin, and that the expression of fibronectin was increased by TGF-beta 1. AP-16 cells should be useful for studying the roles of TGF-beta 1 in the differentiation of astrocyte progenitors.
通过在无血清条件下多次传代，从新生小鼠大脑获得的神经胶质细胞培养物中建立了一种永生化克隆细胞系（AP-16）。免疫荧光实验表明，AP-16细胞表达神经胶质祖细胞标志物（A2B5），不表达少突胶质细胞标志物（半乳糖脑苷脂）或成熟星形胶质细胞标志物（胶质纤维酸性蛋白：GFAP）。用转化生长因子-β1（TGF-β1）或胎牛血清（FCS）处理2天可诱导AP-16细胞分化为A2B5阴性、GFAP阳性、表型成熟的星形胶质细胞。AP-16细胞的存活依赖于表皮生长因子，其生长受到FCS的抑制。这些结果表明，AP-16细胞保留了星形胶质细胞祖细胞的特性。酶联免疫吸附测定表明，AP-16细胞合成纤连蛋白和层粘连蛋白，并且TGF-β1可增加纤连蛋白的表达。AP-16细胞对于研究TGF-β1在星形胶质细胞祖细胞分化中的作用应该是有用的。
