Cobaleda César
Departamento de Fisiología y Farmacología, Universidad de Salamanca, Salamanca, Spain.
Methods Mol Biol. 2010;636:233-50. doi: 10.1007/978-1-60761-691-7_15.
Cellular reprogramming is an interplay between the original starting cell's plasticity and the (epi)genetic mechanisms used to drive this cell towards a new fate. Our capacity to reprogram mature cells into progenitors thus greatly depends on the inherent physiological plasticity of the initial cell. B lymphocytes possess a high degree of plasticity revealed both during their normal development and under experimental conditions in the laboratory. In this chapter, we discuss the biology of B cell plasticity in the context of physiology and pathology and we provide a specific practical example of this plasticity in a protocol describing the dedifferentiation of mature B cells into multipotential progenitors that can afterwards be reprogrammed into alternative lineages like T cells or macrophages.
细胞重编程是原始起始细胞的可塑性与用于驱动该细胞走向新命运的（表观）遗传机制之间的相互作用。因此，我们将成熟细胞重编程为祖细胞的能力在很大程度上取决于初始细胞固有的生理可塑性。B淋巴细胞在其正常发育过程以及实验室实验条件下均表现出高度的可塑性。在本章中，我们将在生理学和病理学背景下讨论B细胞可塑性的生物学特性，并在一个方案中提供这种可塑性的具体实例，该方案描述了成熟B细胞去分化为多能祖细胞的过程，这些祖细胞随后可被重编程为其他谱系，如T细胞或巨噬细胞。
