Wang Yu, Zhang Zai-zhong, Chen Shao-quan, Zou Zhong-dong, Tu Xiao-huang, Wang Lie
Institute of General Surgery, Fuzhou General Hospital, Fuzhou 350025, China.
Zhonghua Wei Chang Wai Ke Za Zhi. 2010 Mar;13(3):219-22.
To evaluate the protective effect of N-acetylcysteine (NAC) on the intestinal barrier dysfunction in rats after extensive abdominal radiation with X ray.
Twenty-four Spraque-Dawley male rats were divided into normal control group (n=8), radiation group (n=8), and radiation+NAC group (300 mg/kg) (n=8). Radiation injury was induced by X ray with a single dose of 10 Gy. NAC was administered from 4 days before irradiation to 3 days after radiation. Three days after radiation, all the rats were euthanized. The terminal ileum was collected for crypt survival assay and ileal villi count. The tissue samples from mesenteric lymph nodes (MLN), spleen, and liver were harvested under sterile conditions for microbiological analysis and ileum samples were harvested for biochemical analysis. The blood levels of D-lactate, endotoxin and diamine oxidase (DAO) and the ileum samples levels of nitric oxide(NO) were also measured.
Rats in radiation+NAC group had a higher survival rate of intestinal crypt [(76.84+/-4.82)% vs (49.64+/-5.48)%, P<0.01], higher intestinal villus count [(8.56+/-0.68)/mm vs (4.02+/-0.54)/mm, P<0.01], lower NO concentration [(0.48+/-0.12) mumol/g vs (0.88+/-0.16) mumol/g, P<0.01], lower levels of D-lactate, endotoxin and DAO (P<0.05 or P<0.01), and significantly decreased enteric bacteria cultured from mesenteric lymph nodes and other tissues as compared with the radiation group (P<0.05 or P<0.01).
NAC protects the small intestine from radiation-induced injury maybe through the inhibition of NO in rats.
评估N-乙酰半胱氨酸（NAC）对X射线腹部大面积照射后大鼠肠道屏障功能障碍的保护作用。
将24只雄性Spraque-Dawley大鼠分为正常对照组（n = 8）、辐射组（n = 8）和辐射+NAC组（300 mg/kg）（n = 8）。采用单次10 Gy的X射线诱导辐射损伤。NAC于照射前4天至照射后3天给药。辐射后3天，将所有大鼠安乐死。收集末端回肠用于隐窝存活率测定和回肠绒毛计数。在无菌条件下采集肠系膜淋巴结（MLN）、脾脏和肝脏的组织样本用于微生物学分析，采集回肠样本用于生化分析。还测量了血液中D-乳酸、内毒素和二胺氧化酶（DAO）水平以及回肠样本中一氧化氮（NO）水平。
辐射+NAC组大鼠的肠道隐窝存活率更高[（76.84±4.82）%对（49.64±5.48）%，P < 0.01]，肠道绒毛计数更高[（8.56±0.68）/mm对（4.02±0.54）/mm，P < 0.01]，NO浓度更低[（0.48±0.12）μmol/g对（0.88±0.16）μmol/g，P < 0.01]，D-乳酸、内毒素和DAO水平更低（P < 0.05或P < 0.01），与辐射组相比，肠系膜淋巴结和其他组织培养的肠道细菌明显减少（P < 0.05或P < 0.01）。
NAC可能通过抑制大鼠体内的NO来保护小肠免受辐射诱导的损伤。
