Research Institute of Surgery, Daping Hospital, Third Military Medical University, Chongqing, China.
J Biomed Mater Res A. 2010 Sep 1;94(3):769-80. doi: 10.1002/jbm.a.32727.
PLGA is thought to be a promising material for nerve scaffold. OECs have been shown to promote axon outgrowth and myelination following peripheral nerve transection. This study assessed the compatibility between PLGA and OECs in vitro, and evaluated the effect of PLGA conduit filled with OECs and extracellular matrix gel (ECM) (POE group) on 10 mm-defect sciatic nerve of rats. Silicon-OECs-EMC (SOE group), PLGA-ECM (PE group), and silicon-ECM (SE group)-were used as the controls. The survival and distribution of OECs in vivo, neurohistology and neurofunction of the bridged nerve, were quantitatively evaluated from 1 week to 12 weeks after surgery. PLGA possessed complete compatibility with OECs. After implantation, OECs migrated along the axis of the nerve and survived longer in the POE group than in the SOE group. Gross recovery of the animal, like ulcerious and autophagical rate as well as relative diameter recovery rate of the fiber, was more successful in the POE group than in other groups. The number of the fiber in the middle and distal segments of bridged sites and neurons in anterior horn of the spinal cord was increased in both OECs-contained groups, but the diameter and the myeline thickness of the fiber were increased only in the POE group. The nerve conduction velocity and the amplitude of compound muscle active potential were improved much successfully in the PLGA-guided group than in the silicon-guided group, but the best improvement was encountered in the POE group. Sciatic function index was not improved in all groups at 12 weeks after surgery due to the injury model. These results suggested that PLGA filled with OECs is a significant alternative to conventional autograft in repairing peripheral nerve defects, and OECs are potential seed cells for peripheral nerve tissue engineering.
聚乳酸-乙醇酸共聚物（PLGA）被认为是一种有前途的神经支架材料。已经证明，嗅鞘细胞（OECs）可以促进周围神经切断后的轴突生长和髓鞘形成。本研究评估了 PLGA 与 OECs 在体外的相容性，并评估了填充 OECs 和细胞外基质凝胶（ECM）的 PLGA 导管（POE 组）对大鼠 10mm 神经缺损的影响。硅-OECs-ECM（SOE 组）、PLGA-ECM（PE 组）和硅-ECM（SE 组）被用作对照组。从手术后 1 周到 12 周，对体内 OECs 的存活和分布、桥接神经的神经组织学和神经功能进行了定量评估。PLGA 与 OECs 完全相容。植入后，OECs 沿神经轴迁移，在 POE 组中的存活时间长于 SOE 组。POE 组动物的大体恢复情况（如溃疡和自噬率以及纤维相对直径恢复率）优于其他组。桥接部位中间和远端段的纤维数量以及脊髓前角的神经元数量在含 OECs 的两组中均增加，但纤维的直径和髓鞘厚度仅在 POE 组中增加。在 PLGA 引导组中，神经传导速度和复合肌肉动作电位的幅度改善明显优于硅引导组，但在 POE 组中改善最为明显。由于损伤模型，所有组在手术后 12 周时坐骨神经功能指数均未改善。这些结果表明，填充 OECs 的 PLGA 是修复周围神经缺损的传统自体移植物的一种有意义的替代方法，OECs 是周围神经组织工程的潜在种子细胞。
