Department of Orthopedic Surgery, College of Medicine, Incheon St. Mary's Hospital, The Catholic University of Korea, 56 Dongsu-ro, Bupyeong-gu, Incheon, 21431, Korea.
Department of Otolaryngology, College of Medicine, Ewha Womans University Seoul Hospital, Ewha Womans University, Seoul, Korea.
Tissue Eng Regen Med. 2021 Jun;18(3):453-465. doi: 10.1007/s13770-020-00326-9. Epub 2021 Jan 30.
Autologous nerve grafts are the gold standard treatment for peripheral nerve injury treatment. However, this procedure cannot avoid sacrificing other nerves as a major limitation. The aim of the present study was to evaluate the potential of olfactory ensheathing cells (OECs) embedded in a nerve conduit.
A 10-mm segment of the sciatic nerve was resected in 21 rats, and the nerve injury was repaired with one of the following (n = 7 per group): autologous nerve graft, poly (ε-caprolactone) (PCL) conduit and OECs, and PCL conduit only. The consequent effect on nerve regeneration was measured based on the nerve conduction velocity (NCV), amplitude of the compound muscle action potential (ACMAP), wet muscle weight, histomorphometric analysis, and nerve density quantification.
Histomorphometric analysis revealed nerve regeneration and angiogenesis in all groups. However, there were significant differences (p < 0.05) in the ACMAP nerve regeneration rate of the gastrocnemius and tibialis anterior muscles between the autologous graft (37.9 ± 14.3% and 39.1% ± 20.4%) and PCL only (17.8 ± 8.6% and 13.6 ± 5.8%) groups, and between the PCL only and PCL + OECs (46.3 ± 20.0% and 34.5 ± 14.6%) groups, with no differences between the autologous nerve and PCL + OEC groups (p > 0.05). No significant results in NCV, wet muscle weight, and nerve density quantification were observed among the 3 groups.
A PCL conduit with OECs enhances the regeneration of injured peripheral nerves, offering a good alternative to autologous nerve grafts.
自体神经移植是周围神经损伤治疗的金标准。然而，该方法存在一个主要局限性，即不可避免地需要牺牲其他神经。本研究旨在评估包埋在神经导管中的嗅鞘细胞（OEC）的潜力。
21 只大鼠切除 10mm 坐骨神经段，采用以下方法之一修复神经损伤（每组 n=7）：自体神经移植、聚己内酯（PCL）导管和 OECs、以及仅 PCL 导管。根据神经传导速度（NCV）、复合肌肉动作电位（ACMAP）幅度、湿重肌肉、组织形态计量分析和神经密度定量来测量神经再生的后续影响。
组织形态计量分析显示所有组均有神经再生和血管生成。然而，在比目鱼肌和胫骨前肌的 ACMAP 神经再生率方面，自体移植物（37.9±14.3%和 39.1%±20.4%）和仅 PCL 组（17.8±8.6%和 13.6%±5.8%）之间以及仅 PCL 组和 PCL+OEC 组（46.3±20.0%和 34.5±14.6%）之间存在显著差异（p<0.05），而自体神经和 PCL+OEC 组之间没有差异（p>0.05）。NCV、湿重肌肉和神经密度定量方面，3 组之间没有显著结果。
PCL 导管加 OEC 可增强受损周围神经的再生，是自体神经移植的良好替代品。
