Department of Orthopaedic Surgery, Hokkaido University, Graduate School of Medicine, Sapporo, Japan.
J Biomed Mater Res A. 2010 Sep 1;94(3):844-55. doi: 10.1002/jbm.a.32762.
We developed a novel cellular implantation system using an in situ forming ultra-purified alginate gel with quite low endotoxity. The aims of this study were to determine the superiority of chondrogenic potential of bone marrow stromal cells (BMSCs) cultured in the purified alginate gel compared with a commercial grade gel, and to assess reparative tissues treated with BMSCs implanted using the developed system into cartilage defects in rabbit knees. The effects of each alginate gel on cellular proliferation and chondrogenesis of rabbit BMSCs were determined by in vitro assessments. Using our purified alginate gel, a novel vehicle system for injecting BMSCs into osteochondral defects without periosteal patch was successfully established in this animal models. The in vitro analyses demonstrated that the purification of alginate significantly enhanced the cellular proliferation and chondrogenic differentiation of BMSCs. The in vivo assessments suggested that the implantation of BMSCs with the developed system using the purified alginate gel histologically and mechanically improved the reparative tissue of osteochondral defects. This system using the purified alginate gel shows the clinical potential for arthroscopically injectable implantation of BMSCs for the treatment of cartilaginous lesions.
我们开发了一种新型细胞植入系统，使用原位形成的超低内毒素的超纯藻酸盐凝胶。本研究的目的是确定在纯化的藻酸盐凝胶中培养的骨髓基质细胞（BMSCs）的软骨形成潜力优于商业级凝胶，并评估使用开发的系统植入 BMSCs 到兔膝关节软骨缺陷中的修复组织。通过体外评估确定了每种藻酸盐凝胶对兔 BMSCs 细胞增殖和软骨形成的影响。使用我们的纯化藻酸盐凝胶，在该动物模型中成功建立了一种新型的将 BMSCs 注射到骨软骨缺陷的注射载体系统，无需骨膜贴片。体外分析表明，藻酸盐的纯化显著增强了 BMSCs 的细胞增殖和软骨分化。体内评估表明，使用开发的系统将 BMSCs 植入纯化藻酸盐凝胶在组织学和力学上改善了骨软骨缺陷的修复组织。该系统使用纯化的藻酸盐凝胶显示出临床潜力，可用于关节镜下注射植入 BMSCs 治疗软骨病变。
