Norming U, Nyman C R, Tribukait B
Department of Urology, South Hospital, Stockholm, Sweden.
J Urol. 1991 Jun;145(6):1164-8. doi: 10.1016/s0022-5347(17)38563-4.
In a study of 290 patients with untreated carcinoma of the bladder, histopathological studies of random mucosal biopsies were compared with the results of deoxyribonucleic acid (DNA) flow cytometry. By histopathology the findings were classified as severe atypia corresponding to primary grade 3 carcinoma in situ, atypia not fulfilling the criteria for carcinoma in situ and no atypia. The DNA histograms were classified as diploid or aneuploid. Aneuploid cell populations in mucosal biopsies were found mainly in cases with aneuploid tumors of grade 3. Of the biopsies classified as concomitant carcinoma in situ 76% were aneuploid. In biopsies exhibiting less severe or no atypia aneuploidy was found in 41 and 10%, respectively. For these 3 morphological categories the distributions of the aneuploid cell populations were similar irrespective of the histopathological findings and they were also the same as that found in primary carcinoma in situ. We concluded that gross chromosomal aberrations may appear at an early stage of the tumor development and before changes recognizable by morphology. The similarity of the DNA profiles of the aneuploid cell populations, regardless of morphological findings, indicates that apart from gross chromosomal aberrations changes of the phenotype are necessary for the expression of morphological changes.
在一项对290例未经治疗的膀胱癌患者的研究中，将随机黏膜活检的组织病理学研究结果与脱氧核糖核酸（DNA）流式细胞术的结果进行了比较。通过组织病理学，将结果分类为对应于原发性3级原位癌的重度异型增生、不符合原位癌标准的异型增生以及无异型增生。DNA直方图被分类为二倍体或非整倍体。黏膜活检中的非整倍体细胞群主要见于3级非整倍体肿瘤病例。在分类为伴发原位癌的活检中，76%为非整倍体。在显示异型增生较轻或无异型增生的活检中，非整倍体分别占41%和10%。对于这3种形态学类别，无论组织病理学结果如何，非整倍体细胞群的分布都是相似的，并且与原发性原位癌中的分布相同。我们得出结论，染色体畸变可能在肿瘤发展的早期阶段以及形态学可识别的变化之前出现。非整倍体细胞群的DNA图谱相似，无论形态学结果如何，这表明除了染色体畸变外，表型变化对于形态学变化的表达也是必要的。
