Shaaban A A, Tribukait B, el-Bedeiwy A F, Ghoneim M A
Urology and Nephrology Centre, Mansoura University, Egypt.
J Urol. 1990 Oct;144(4):879-83. doi: 10.1016/s0022-5347(17)39614-3.
We studied 100 cases of squamous cell carcinoma of the bladder by flow cytometry after cystectomy. Tumors were classified according to the deoxyribonucleic acid profile into diploid or aneuploid. Proliferation of the tumors was assessed from the proportions of S-phase cells. The flow cytometric data were correlated to the histopathological stage and grade. Grade 1 tumors could be subdivided into diploid and aneuploid in 60 and 40% of the cases, respectively, while 95% of the grade 2 and all grade 3 tumors were aneuploid. Diploid tumors had low proliferation rates, while aneuploid tumors had significantly higher values. A high frequency of muscle invasive diploid squamous cell tumors was noted. Tumor heterogeneity was studied by comparing cell material from superficial and deep tumor areas, which were in agreement in 77% of the cases. By comparing biopsy material with that obtained by bladder washings, biopsy material yielded better information regarding deoxyribonucleic acid ploidy in half of the aneuploid tumors. These results indicate that flow cytometry offers an additional objective method to characterize squamous cell carcinoma.
我们在膀胱切除术后通过流式细胞术研究了100例膀胱鳞状细胞癌。根据脱氧核糖核酸谱将肿瘤分为二倍体或非整倍体。根据S期细胞比例评估肿瘤的增殖情况。将流式细胞术数据与组织病理学分期和分级相关联。1级肿瘤分别有60%和40%可分为二倍体和非整倍体，而2级肿瘤的95%和所有3级肿瘤均为非整倍体。二倍体肿瘤增殖率低，而非整倍体肿瘤的值明显更高。注意到肌肉浸润性二倍体鳞状细胞瘤的频率较高。通过比较肿瘤浅表和深部区域的细胞材料研究肿瘤异质性，77%的病例结果一致。通过将活检材料与膀胱冲洗获得的材料进行比较，活检材料在一半的非整倍体肿瘤中提供了关于脱氧核糖核酸倍性的更好信息。这些结果表明，流式细胞术为膀胱鳞状细胞癌的特征化提供了一种额外的客观方法。
