Liu Xiudong
College of Environment and Chemical Engineering, Dalian University, Dalian 116622, China.
Sheng Wu Yi Xue Gong Cheng Xue Za Zhi. 2010 Feb;27(1):97-101.
Small interference RNA (siRNA) induced RNA interference (RNAi) technology has shown high specificity and high efficiency of silencing target gene expression, and it is becoming a promising candidate drug for the therapy of cancer and viral infection diseases. At present, the lack of safe and effective carrier materials and delivery systems of siRNA through extracellular and intracellular barriers still hampers the clinical application. In order to overcome this difficulty, we proposed using chitosan, naturally occurring polycation, to form complex siRNA against green fluorescence protein (siRNA-eGFP). The spherical and stable chitosan-siRNA nanoparticles with 83%-94% siRNA complex efficiency can be formulated under mild electrostatic interaction. The size and Zeta potential of nanoparticles were within the range of 90-180 nm and 10-30 mV, respectively. 80% cell viability could be maintained inthe course of incubating with chitosan-siRNA nanoparticles. Moreover, nearly 80% gene silencing efficiency of chitosan-siRNA nanoparticles was realized.
小干扰RNA（siRNA）诱导的RNA干扰（RNAi）技术已显示出在沉默靶基因表达方面的高特异性和高效率，并且正成为治疗癌症和病毒感染性疾病的一种有前景的候选药物。目前，缺乏安全有效的载体材料以及siRNA穿过细胞外和细胞内屏障的递送系统仍然阻碍了其临床应用。为了克服这一困难，我们提出使用天然存在的聚阳离子壳聚糖来形成针对绿色荧光蛋白的复合siRNA（siRNA-eGFP）。在温和的静电相互作用下，可以制备出siRNA复合效率为83%-94%的球形且稳定的壳聚糖-siRNA纳米颗粒。纳米颗粒的尺寸和Zeta电位分别在90-180 nm和10-30 mV范围内。在用壳聚糖-siRNA纳米颗粒孵育过程中可维持80%的细胞活力。此外，壳聚糖-siRNA纳米颗粒实现了近80%的基因沉默效率。
