Jiang Shicui, Wang Yi, Li Xiaokun, Han Xiuwen, Zhang Meiping
College of Life Sciences, Jilin Agriculture University, Changchun 130118, China.
Sheng Wu Yi Xue Gong Cheng Xue Za Zhi. 2010 Feb;27(1):126-31.
Acid fibroblast growth factor (aFGF) has great potential in clinical application, but it is very expensive. In order to reduce the cost of production and to make full use of the merits integrated with plant bioreator, we have explored the aFGF in transgenic Tobacco expression. AFGF gene was inserted into plant expression vector pBI121; the acquired plants contained aFGF gene expression vector pBI121-TOAB-aF. Using Agrobacterium-mediated gene transformation of Tobacco and using transgenic Tobacco containing kanamycin and cephalosporin culture medium, we obtained kanamycin resistant transgenic Tobacco plants. PCR detection, RT-PCR detection and Western blot detection confirmed that foreign genes were successfully expressed in Tobacco. These data could serve as a theoretical foundation on which to use the plant bioreactor for production of aFGF.
酸性成纤维细胞生长因子（aFGF）在临床应用中有很大潜力，但成本很高。为了降低生产成本并充分利用植物生物反应器的优点，我们探索了aFGF在转基因烟草中的表达。将AFGF基因插入植物表达载体pBI121；获得的植物含有aFGF基因表达载体pBI121-TOAB-aF。利用农杆菌介导的烟草基因转化，并使用含有卡那霉素和头孢菌素的培养基培养转基因烟草，我们获得了抗卡那霉素的转基因烟草植株。PCR检测、RT-PCR检测和Western blot检测证实外源基因在烟草中成功表达。这些数据可为利用植物生物反应器生产aFGF提供理论依据。
