Hu Yao-hui, Liu Bei-bei, Yu Han-song
College of Food Science and Engineering, Jilin Agricultural University, Changchun 130118, China.
Zhong Yao Cai. 2011 Jun;34(6):864-8.
To construct root-specific promoter of plant expression vector and transformate into Rhodiola hairy root.
The expression vector pCA-Tob7: UGTR, driven by tobacco root-specific promoter TobRB7, was constructed from pCAM-BIA1301 by substituting the CaMV 35S promoter and GUS gene with TobRB7 and UGTR(a glycosyltransferase gene), respectively. The pCA-Tob7: UGTR vector was introduced into Agrobacterium tumefaciens strain, and the hairy root of Rhodiola were then transformed.
Some Kanamycin resistant plants were positive,which indicated that the expression vector had integrated into Rhodiola hairy root genome.
Root-specific promoter of plant expression vector is constructed and the hairy root of Rhodiola are transformed for future research.
构建植物表达载体的根特异性启动子并转化至红景天毛状根中。
以pCAM - BIA1301为基础，分别用烟草根特异性启动子TobRB7和UGTR（一种糖基转移酶基因）替换CaMV 35S启动子和GUS基因，构建由烟草根特异性启动子TobRB7驱动的表达载体pCA - Tob7: UGTR。将pCA - Tob7: UGTR载体导入根癌农杆菌菌株，然后转化红景天毛状根。
一些卡那霉素抗性植株呈阳性，表明表达载体已整合到红景天毛状根基因组中。
构建了植物表达载体的根特异性启动子，并对红景天毛状根进行了转化，以供未来研究使用。
