The First Affiliated Hospital of Zhengzhou University, Zhengzhou 450052Z Henan, China.
Biomed Environ Sci. 2009 Dec;22(6):522-8. doi: 10.1016/S0895-3988(10)60011-6.
To characterize the human immunodeficiency virus (HIV) -specific T lymphocyte responses and identify the immunodominant regions in Chinese HIV-1 recombinant subtype B/C chronic infectors at complete genome level.
Twenty-five HIV-1B/C recombinant chronic infectors were screened for their specific T lymphocyte responses to a panel of peptides corresponding to the complete HIV-1 subtype B genome by gamma interferon ELISPOT assay. Kruskal-Wallis nonparametric analysis of variance was used to test significant differences across gene regions, and Tukey pairwise analysis was used to identify differences between gene regions. Spearman rank correlation was used to assess the relation between responses. Results The order of recognized frequencies of specific T lymphocyte responses to HIV proteins was Nef>Vpr>Gag>Pol>Vpu>Env>Rev>Vif>Tat. When adjusted for protein length, Nef, Vpr, Gag, and Pol were the most intensely targeted proteins and the central region of Nef, Gag p24, Pol RT, and Vpr was most frequently recognized. No significant correlation was observed between the magnitude of IFN-gamma production of HIV-l-specific T lymphocyte responses and plasma viremia, breadth of response and CD4 counts. Conclusion The central region of Nef, Gag p24, Pol RT, and Vpr is most frequently targeted in HIV-1 B/C recombinants chronic infectors. HIV-l-specific T lymphocyte responses and plasma viremia or CD4 counts play no protective role at complete genome level in these infectors.
在完整基因组水平上，描绘中国 HIV-1 重组亚型 B/C 慢性感染者的 HIV 特异性 T 淋巴细胞反应，并确定免疫优势区域。
通过γ干扰素 ELISPOT 测定，对 25 例 HIV-1B/C 重组慢性感染者进行了针对完整 HIV-1 亚型 B 基因组的肽段的特异性 T 淋巴细胞反应的筛选。采用 Kruskal-Wallis 非参数方差分析检验基因区域之间的显著差异，采用 Tukey 两两分析确定基因区域之间的差异。采用 Spearman 秩相关评估反应之间的关系。
对 HIV 蛋白的特异性 T 淋巴细胞反应的识别频率的顺序为 Nef>Vpr>Gag>Pol>Vpu>Env>Rev>Vif>Tat。当根据蛋白长度进行调整时，Nef、Vpr、Gag 和 Pol 是最受关注的蛋白，而 Nef 的中央区域、Gag p24、Pol RT 和 Vpr 是最常被识别的区域。HIV-1 特异性 T 淋巴细胞反应的 IFN-γ产生量与血浆病毒载量、反应广度和 CD4 计数之间未观察到显著相关性。
在 HIV-1B/C 重组慢性感染者中，Nef、Gag p24、Pol RT 和 Vpr 的中央区域是最常被攻击的目标。在这些感染者中，HIV-1 特异性 T 淋巴细胞反应和血浆病毒载量或 CD4 计数在完整基因组水平上没有发挥保护作用。
