INRS-Institut Armand-Frappier, Institut National de la Recherche Scientifique, Laval, Québec, Canada H7V 1B7.
Annu Rev Phytopathol. 2010;48:69-91. doi: 10.1146/annurev-phyto-073009-114239.
This review focuses on the extensive membrane and organelle rearrangements that have been observed in plant cells infected with RNA viruses. The modifications generally involve the formation of spherules, vesicles, and/or multivesicular bodies associated with various organelles such as the endoplasmic reticulum and peroxisomes. These virus-induced organelles house the viral RNA replication complex and are known as virus factories or viroplasms. Membrane and organelle alterations are attributed to the action of one or two viral proteins, which additionally act as a scaffold for the assembly of a large complex of proteins of both viral and host origin and viral RNA. Some virus factories have been shown to align with and traffic along microfilaments. In addition to viral RNA replication, the factories may be involved in other processes such as viral RNA translation and cell-to-cell virus transport. Confining the process of RNA replication to a specific location may also prevent the activation of certain host defense functions.
本综述重点介绍了感染 RNA 病毒的植物细胞中观察到的广泛的膜和细胞器重排。这些修饰通常涉及形成与内质网和过氧化物酶体等各种细胞器相关的球体、小泡和/或多泡体。这些病毒诱导的细胞器容纳病毒 RNA 复制复合物，被称为病毒工厂或病毒质体。膜和细胞器的改变归因于一种或两种病毒蛋白的作用，这些蛋白还充当组装大量病毒和宿主来源的蛋白质以及病毒 RNA 的大型复合物的支架。已经证明一些病毒工厂与微丝对齐并沿其运输。除了病毒 RNA 复制外，这些工厂可能还参与其他过程，如病毒 RNA 翻译和细胞间病毒运输。将 RNA 复制过程限制在特定位置也可能防止某些宿主防御功能的激活。
