Clinical Research Center, the First Affiliated Hospital of Nanjing Medical University, Nanjing, Jiangsu, China.
Allergy. 2010 Oct;65(10):1234-41. doi: 10.1111/j.1398-9995.2010.02349.x. Epub 2010 Mar 20.
The role of interleukin (IL)-29 in innate immunity has been recognized recently, and it is regarded as a potent bioactive molecule. However, little is known about its role in the pathogenesis of allergy. Because mast cells are recognized as primary effector cells of allergy, we investigated the potential relationship between IL-29 and mast cells in this study.
To examine the expression of IL-29 in mast cells and the influence of IL-29 on mast cell mediator release and accumulation.
Expression of IL-29 in mast cells was determined by double-labeling immunohistochemistry and flow cytometry analysis. Mast cell cell-line was cultured to examine the mediator release, and mouse peritoneal model was employed to observe the mast cell accumulation.
Large proportions of mast cells expressing IL-29 were localized in human tissue including the colon, tonsil and lung. Mast cells can release substantial quantity of IL-29 upon challenge with proteolytic allergens. Extrinsic IL-29 provoked IL-4 and IL-13 release from mast cell line P815 cells through PI3K/Akt and (JAK)/STAT3 signaling pathways, but failed to induce mast cell histamine release from human mast cells. Extrinsic IL-29 also induced mast cell infiltration in mouse peritoneum by a CD18- and ICAM1-dependent mechanism.
Mast cell-derived IL-29 has the potential to be involved in the pathogenesis of allergic inflammation.
白细胞介素（IL）-29 在先天免疫中的作用最近才被认识到，它被认为是一种有效的生物活性分子。然而，其在过敏发病机制中的作用知之甚少。由于肥大细胞被认为是过敏的主要效应细胞，我们在这项研究中研究了 IL-29 与肥大细胞之间的潜在关系。
检测 IL-29 在肥大细胞中的表达及其对肥大细胞介质释放和积累的影响。
通过双标记免疫组织化学和流式细胞术分析检测 IL-29 在肥大细胞中的表达。培养肥大细胞系以检测介质释放，并采用小鼠腹腔模型观察肥大细胞积累。
大量表达 IL-29 的肥大细胞定位于包括结肠、扁桃体和肺在内的人体组织中。肥大细胞在受到蛋白水解过敏原刺激时会释放大量的 IL-29。外源性 IL-29 通过 PI3K/Akt 和 (JAK)/STAT3 信号通路从肥大细胞系 P815 细胞中引发 IL-4 和 IL-13 的释放，但不能诱导人肥大细胞释放组胺。外源性 IL-29 还通过 CD18 和 ICAM1 依赖的机制诱导小鼠腹腔内的肥大细胞浸润。
肥大细胞衍生的 IL-29 有可能参与过敏炎症的发病机制。
