Department of Oral Surgery, Medical University of Vienna, Vienna, Austria.
Clin Oral Implants Res. 2010 Jun;21(6):656-61. doi: 10.1111/j.1600-0501.2009.01888.x.
Collagen barrier membranes (CBM) are used for guided bone regeneration to support the process of graft consolidation. It remains, unknown however, whether CBM can affect the consolidation of bone grafts by controlling the differentiation of progenitor cells into bone-resorbing osteoclasts and bone-forming osteoblasts.
To gain an insight into the underlying mechanisms, we performed in vitro bone marrow cultures on CBM (Bio-Gide under conditions that favor osteoclastogenesis and osteoblastogenesis, respectively. Measures of osteoclastogenesis were based on the number of tartrate-resistant acid-phosphatase-positive (TRAP+) multinucleated cells. Resorption assays revealed the activity of mature osteoclasts. Osteoblastogenesis was determined by alkaline-phosphatase activity. Viability was investigated utilizing the MTT assay.
Cultivation of murine bone marrow on CBM reduced the number of TRAP+ multinucleated cells compared with cultures on tissue culture plates. Inhibition of osteoclastogenesis was observed on the porous and the dense CBM surfaces. The majority of TRAP+ cells were mononucleated and the decreased osteoclastogenesis was not due to changes in cell viability. Furthermore, CBM are inert regarding the resorptive activity of mature osteoclasts. Moreover, osteoblastogenesis was not reduced when bone marrow cells were grown on the surface of CBM.
These in vitro findings demonstrate that CBM can reduce the formation but not the activity of multinucleated osteoclasts. Our data further reveal that the formation of osteogenic cells from their progenitors is not reduced by the CBM. Overall, our results suggest that the beneficial effects of CBM during graft consolidation may involve their direct impact on osteoclastogenesis.
胶原屏障膜（CBM）用于引导骨再生，以支持移植物整合过程。然而，CBM 是否可以通过控制祖细胞分化为破骨细胞和成骨细胞来影响骨移植物的整合仍不清楚。
为了深入了解潜在机制，我们在 CBM（Bio-Gide）上进行了体外骨髓培养，分别有利于破骨细胞生成和成骨细胞生成的条件。破骨细胞生成的测量基于抗酒石酸酸性磷酸酶阳性（TRAP+）多核细胞的数量。吸收测定揭示了成熟破骨细胞的活性。成骨细胞生成通过碱性磷酸酶活性确定。利用 MTT 测定法研究了细胞活力。
与在组织培养板上培养相比，在 CBM 上培养的鼠骨髓中 TRAP+多核细胞的数量减少。在多孔和致密 CBM 表面观察到破骨细胞生成受到抑制。大多数 TRAP+细胞是单核细胞，破骨细胞生成减少不是由于细胞活力的变化。此外，CBM 对成熟破骨细胞的吸收活性是惰性的。此外，当骨髓细胞在 CBM 表面生长时，成骨细胞生成没有减少。
这些体外发现表明 CBM 可以减少多核破骨细胞的形成，但不能减少其活性。我们的数据进一步表明，CBM 不会减少其祖细胞形成成骨细胞的能力。总的来说，我们的结果表明，CBM 在移植物整合过程中的有益作用可能涉及其对破骨细胞生成的直接影响。
