Department of Plant and Soil Science, School of Biological Sciences, University of Aberdeen, Aberdeen, UK.
J Appl Microbiol. 2010 Sep;109(3):829-38. doi: 10.1111/j.1365-2672.2010.04711.x.
To assess whether flies and slugs acquire strains of Campylobacter jejuni and Campylobacter coli present in local ruminant faeces.
Campylobacter was cultured from flies, slugs and ruminant faeces that were collected from a single farm in Scotland over a 19-week period. The isolates were typed using multi-locus sequence typing (MLST) and compared with isolates from cattle and sheep faeces. Campylobacter jejuni and Camp. coli were isolated from 5·8% (n=155, average of 75 flies per pool) and 13·3% (n=15, average of 8·5 slugs per pool) of pooled fly and slug samples, respectively. The most common sequence type (ST) in flies was Camp. coli ST-962 (approx. 40%) regardless of the prevalence in local cattle (2·3%) or sheep (25·0%) faeces. Two positive slug pools generated the same ST that has not been reported elsewhere.
Despite their low carriage rate, flies are able to acquire Campylobacter STs that are locally present, although the subset carried may be biased when compared to local source. Slugs were shown to carry a previously unreported Campylobacter ST.
This study has demonstrated that flies carry viable Campylobacter and may contribute to the transfer of STs within and between groups of animals on farms. Further, they may therefore present a risk to human health via their contact with ready-to-eat foods or surfaces.
评估苍蝇和鼻涕虫是否会从当地反刍动物粪便中获得存在的空肠弯曲菌和大肠弯曲菌菌株。
在苏格兰的一个农场，我们在 19 周的时间内收集苍蝇、鼻涕虫和反刍动物粪便，并从中培养弯曲菌。我们使用多位点序列分型（MLST）对分离株进行分型，并将其与牛和羊粪便中的分离株进行比较。从苍蝇和鼻涕虫混合样本中分别分离出 5.8%（n=155，每个样本平均有 75 只苍蝇）和 13.3%（n=15，每个样本平均有 8.5 只鼻涕虫）的空肠弯曲菌和大肠弯曲菌。苍蝇中最常见的序列型（ST）是空肠弯曲菌 ST-962（约 40%），而无论当地牛（2.3%）或羊（25.0%）粪便中的流行情况如何。两个阳性鼻涕虫样本产生了一种尚未在其他地方报道过的 ST。
尽管携带率低，但苍蝇能够获得当地存在的弯曲菌 ST，尽管与当地来源相比，携带的亚群可能存在偏差。鼻涕虫携带了一种以前未报道过的弯曲菌 ST。
本研究表明，苍蝇携带有活力的弯曲菌，可能会导致农场动物群体内部和群体之间的 ST 转移。此外，它们可能会通过接触即食食品或表面而对人类健康构成威胁。
