Section of Immunology and Infection, School of Medicine and Dentistry, University of Aberdeen, Foresterhill, Aberdeen, United Kingdom.
Appl Environ Microbiol. 2011 Sep;77(18):6579-86. doi: 10.1128/AEM.00428-11. Epub 2011 Jul 22.
Campylobacter jejuni and C. coli were quantified and typed, using multilocus sequence typing (MLST), from fecal samples collected from a mixed cattle and sheep farm during summer. Cattle had a significantly higher prevalence than sheep (21.9% [74/338] and 14.0% [30/214], respectively), but both decreased over time. There were no differences in the average Campylobacter concentrations shed by cattle (600 CFU g(-1)) and sheep (820 CFU g(-1)), although sheep did show a significant temporal reduction in the number of Campylobacter organisms shed in their feces. A total of 21 different sequence types (STs) (97.7% C. jejuni, 2.3% C. coli) were isolated from cattle, and 9 different STs were isolated from sheep (40.6% C. jejuni, 59.4% C. coli). The Campylobacter population in cattle was relatively stable, and the frequencies of genotypes isolated showed little temporal variation. However, the composition of subtypes isolated from sheep did show significant temporal differences. The cattle and sheep consistently showed significant differences in their carriage of Campylobacter species, STs, and CCs despite the fact that both were exposed to the same farming environment. This work has highlighted the patterns of a Campylobacter population on a ruminant farm by identifying the existence of both temporal and between-host variations.
从夏季在一个牛羊混合农场采集的粪便样本中，使用多位点序列分型（MLST）对空肠弯曲菌和大肠弯曲菌进行了定量和分型。牛的流行率明显高于绵羊（分别为 21.9%[74/338]和 14.0%[30/214]），但随时间推移而降低。牛（600 CFU g(-1))和绵羊（820 CFU g(-1))粪便中排出的弯曲菌平均浓度没有差异，尽管绵羊粪便中排出的弯曲菌数量确实呈显著的时间减少。从牛中分离出 21 种不同的序列型（ST）（97.7%空肠弯曲菌，2.3%大肠弯曲菌），从绵羊中分离出 9 种不同的 ST（40.6%空肠弯曲菌，59.4%大肠弯曲菌）。牛的弯曲菌种群相对稳定，分离基因型的频率变化不大。然而，从绵羊中分离出的亚型组成确实表现出明显的时间差异。尽管牛和绵羊都暴露在相同的养殖环境中，但它们携带的弯曲菌种类、ST 和 CC 却存在显著差异。这项工作通过确定时间和宿主之间的变异的存在，突出了牛羊养殖场中弯曲菌种群的模式。
