Department of Operative Dentistry and Periodontology, University School of Dental Medicine, Martin-Luther University Halle-Wittenberg, Halle, Germany.
J Periodontal Res. 2010 Jun;45(3):396-403. doi: 10.1111/j.1600-0765.2009.01251.x. Epub 2010 Mar 9.
Periodontitis is influenced by specific host-dependent immune responses. Periodontopathogens induce innate immune responses, amongst others, via toll-like receptor 2 (TLR2), resulting in activation of the nuclear transcription factor nuclear factor-kappaB (NF-kappaB). The aim of this case-control study was to evaluate links between genetic variants of these genes and chronic/aggressive periodontitis in a multivariate model.
A total of 141 patients with periodontitis (63 with chronic periodontitis and 78 with aggressive periodontitis) and 81 controls without periodontitis were included in the study. Polymorphisms in TLR2 (Arg677Trp, Arg753Gln) and in NF-kappaB (-94ins/delATTG) were determined by restriction fragment length polymorphism and fragment length analyses, respectively. Subgingival bacterial colonization was evaluated using a PCR/DNA probe test (micro-Ident).
Although there was no association of the TLR2 polymorphism Arg753Gln with periodontitis, heterozygous carriers (Arg/Gln) were at a higher risk for colonization with bacteria of the 'red complex' (corrected p-value = 0.042). The del/del genotype of the NF-kappaB polymorphism was associated with aggressive periodontitis considering age, gender, smoking and approximal plaque index as potential confounders (odds ratio = 2.81, p = 0.035, 95% confidence interval: 1.08-7.33). del/del carriers had a higher risk for subgingival colonization with Aggregatibacter actinomycetemcomitans (odds ratio = 2.36, p = 0.030, 95% confidence interval: 1.09-5.1; adjusted for age, gender, smoking and pocket depth(bacteria)).
The del/del genotype of NF-kappaB was shown to be associated with the occurrence of aggressive periodontitis.
牙周炎受特定宿主依赖性免疫反应的影响。牙周病原体通过 Toll 样受体 2（TLR2）诱导先天免疫反应，导致核转录因子核因子-κB（NF-κB）激活。本病例对照研究的目的是在多变量模型中评估这些基因的遗传变异与慢性/侵袭性牙周炎之间的联系。
共纳入 141 例牙周炎患者（63 例慢性牙周炎，78 例侵袭性牙周炎）和 81 例无牙周炎对照者。TLR2（Arg677Trp、Arg753Gln）和 NF-κB（-94ins/delATTG）的多态性分别通过限制性片段长度多态性和片段长度分析确定。使用聚合酶链反应/DNA 探针试验（微识别）评估龈下细菌定植情况。
虽然 TLR2 多态性 Arg753Gln 与牙周炎无关，但杂合子携带者（Arg/Gln）更易定植“红色复合体”细菌（校正 p 值=0.042）。考虑到年龄、性别、吸烟和邻面菌斑指数等潜在混杂因素，NF-κB 多态性的 del/del 基因型与侵袭性牙周炎相关（比值比=2.81，p=0.035，95%置信区间：1.08-7.33）。del/del 携带者龈下定植 Aggregatibacter actinomycetemcomitans 的风险更高（比值比=2.36，p=0.030，95%置信区间：1.09-5.1；校正年龄、性别、吸烟和牙周袋深度（细菌））。
NF-κB 的 del/del 基因型与侵袭性牙周炎的发生相关。
