Gastrointestinal Laboratory, Department of Small Animal Clinical Sciences, College of Veterinary Medicine and Biomedical Sciences, Texas A&M University, College Station, TX, USA.
J Vet Intern Med. 2010 May-Jun;24(3):643-6. doi: 10.1111/j.1939-1676.2010.0489.x. Epub 2010 Mar 15.
An assay for the measurement of pancreatic elastase in dog feces has been introduced.
HYPOTHESIS/OBJECTIVES: The goal of this study was to evaluate the rate of false-positive fecal-elastase test results in dogs with suspected exocrine pancreatic insufficiency (EPI) and to assess serum cholecystokinin (CCK) concentrations in dogs with a false positive fecal elastase test result.
Twenty-six fecal and serum samples from dogs suspected of EPI, for which samples had been submitted to a commercial laboratory (Vet Med Labor) for analysis.
Prospective study. Serum trypsin-like immunoreactivity (TLI) was measured in 26 dogs with a decreased fecal elastase concentration of <10 microg/g feces. Serum CCK concentrations were measured in 21 of these dogs.
Of 26 dogs with a decreased fecal elastase concentration, 6 (23%) had serum TLI concentrations within or above the reference range. Serum CCK concentrations were significantly higher in dogs with a true positive fecal elastase test result (median: 1.1 pmol/L; range: 0.1-3.3 pmol/L) than in those with a false positive fecal elastase test result (median: 0.1 pmol/L; range: 0.1-0.9 pmol/L; P value = .0163).
The rate of false positive fecal elastase test results was high in this group of dogs, suggesting that diagnosis of EPI must be confirmed by other means. The decreased CCK concentration in dogs with a false positive fecal elastase test result could suggest that false positive results are because of decreased stimulation of exocrine pancreatic function caused by other conditions.
现已引入用于测量犬粪便中胰弹性蛋白酶的检测方法。
假说/目的：本研究旨在评估疑似胰腺外分泌功能不全（EPI）犬的粪便弹性蛋白酶检测结果假阳性率，并评估粪便弹性酶检测结果假阳性犬的血清胆囊收缩素（CCK）浓度。
26 份疑似 EPI 犬的粪便和血清样本，这些样本曾被提交至一家商业实验室（Vet Med Labor）进行分析。
前瞻性研究。对 26 只粪便弹性酶浓度降低（<10 μg/g 粪便）的犬进行血清胰蛋白酶样免疫反应性（TLI）检测。对其中 21 只犬进行血清 CCK 浓度检测。
在 26 只粪便弹性酶浓度降低的犬中，有 6 只（23%）的血清 TLI 浓度在参考范围内或以上。粪便弹性酶检测结果为真阳性的犬的血清 CCK 浓度显著高于粪便弹性酶检测结果为假阳性的犬（中位数：1.1 pmol/L；范围：0.1-3.3 pmol/L）（P 值=0.0163）。
在这组犬中，粪便弹性酶检测结果假阳性率较高，这表明必须通过其他方法来确认 EPI 的诊断。粪便弹性酶检测结果假阳性犬的 CCK 浓度降低可能表明假阳性结果是由于其他疾病导致外分泌胰腺功能刺激减少所致。
