Spillmann T, Wittker A, Teigelkamp S, Eim C, Burkhardt E, Eigenbrodt E, Sziegoleit A
Medizinische und Gerichtliche Veterinärklinik I, Universität Giessen, Germany.
J Vet Diagn Invest. 2001 Nov;13(6):468-74. doi: 10.1177/104063870101300603.
The detection of pancreatic elastase 1 in stool samples has become the noninvasive gold standard for the diagnosis of pancreatic insufficiency in humans. Accordingly, the development of a sandwich-ELISA specific for canine pancreatic elastase 1, based on monoclonal antibodies, is presented here. The test has a detection range of 4-240 microg canine pancreatic elastase l/g feces. The intraassay coefficient of variation is 7.4%, and the interassay coefficient of variation is 7.7%. Spiking experiments show that canine elastase 1 is quantitatively detectable in fecal samples. Interestingly, the range of the elastase 1 concentration in canine feces within several days is higher as compared with humans. As the proposed cutoff of 10 microg/g is below this variation range in 96.1% of the tested samples, the effect on the test specificity is negligible. Because the test detects neither human nor bovine and porcine elastase 1, pancreatic function can be monitored without interrupting an enzyme replacement therapy.
粪便样本中胰腺弹性蛋白酶1的检测已成为人类胰腺功能不全诊断的非侵入性金标准。因此，本文介绍了一种基于单克隆抗体的、针对犬胰腺弹性蛋白酶1的夹心酶联免疫吸附测定法（sandwich-ELISA）。该检测方法的检测范围为4 - 240微克犬胰腺弹性蛋白酶1/克粪便。批内变异系数为7.4%，批间变异系数为7.7%。加标实验表明，犬弹性蛋白酶1在粪便样本中可进行定量检测。有趣的是，与人类相比，犬粪便中弹性蛋白酶1浓度在几天内的变化范围更高。由于所提议的10微克/克的临界值在96.1%的测试样本中低于此变化范围，因此对检测特异性的影响可忽略不计。由于该检测方法既不检测人、牛也不检测猪的弹性蛋白酶1，所以在不中断酶替代疗法的情况下即可监测胰腺功能。
