Department of Microbiology, West China School of Preclinical and Forensic Medicine, Sichuan University, Chengdu 610041, China.
Lett Appl Microbiol. 2010 May;50(5):468-73. doi: 10.1111/j.1472-765X.2010.02824.x. Epub 2010 Feb 15.
To identify the presence of mouse beta-defensin 3 (Mbd3) (the human homologue of beta-defensin 2) in different tissues and to define the antimicrobial properties of recombinant MBD3 (rMBD3) against a panel of human pathogens.
Mbd3 gene expression in different mouse tissues before or after lipopolysaccharide (LPS) injection was compared by semi-quantitative RT-PCR. This analysis demonstrated that epithelial and mucosal tissues expressed Mbd3 independent of LPS stimulation. Evaluation of the antimicrobial properties of recombinant rMBD3 was determined by assessing the median inhibition concentration (IC(50)), minimal inhibitory concentration (MIC) and minimal bactericidal concentration (MBC)/minimal fungicidal concentration (MFC) against various human pathogens.
Mbd3 gene expression by epithelial and mucosal tissues suggested that MBD3 likely plays an early defensive role against microbial infections. This activity was most significant against filamentous fungi.
The data presented in this report suggested that formulations containing rMBD3 and related molecules could serve to treat fungal and bacterial infections.
鉴定小鼠β-防御素 3（Mbd3）（β-防御素 2 的人类同源物）在不同组织中的存在，并确定重组 MBD3（rMBD3）对一系列人类病原体的抗菌特性。
通过半定量 RT-PCR 比较了不同组织中 LPS 注射前后 Mbd3 基因的表达。该分析表明，上皮和黏膜组织在 LPS 刺激下表达 Mbd3。通过评估重组 rMBD3 的抑菌浓度（IC（50））、最小抑菌浓度（MIC）和最小杀菌浓度（MBC）/最小抑菌浓度（MFC）对各种人类病原体的作用，来确定其抗菌特性。
上皮和黏膜组织中 Mbd3 基因的表达表明 MBD3 可能在针对微生物感染的早期防御中发挥作用。该活性对抗丝状真菌最为显著。
本报告中提供的数据表明，含有 rMBD3 和相关分子的制剂可用于治疗真菌和细菌感染。
