Mycotic Diseases Branch, Division of Food-borne Bacterial and Mycotic Diseases, Centers for Disease Control and Prevention, Atlanta, GA 30333, USA.
Mycoses. 2011 Jul;54(4):e154-62. doi: 10.1111/j.1439-0507.2010.01862.x. Epub 2010 Mar 11.
Candida spp. biofilms can be established on a wide range of materials, including implanted medical devices, and can display a resistant phenotype to antifungal drugs. Several factors, including host and surface properties, may influence the establishment and the development of Candida albicans biofilms on biotic and abiotic surfaces. We therefore selected a collection of C. albicans clinical isolates to evaluate the effect of surface and serum on biofilm attachment and development. Disc coupons from the CDC biofilm reactor were used in a well plate assay to study biofilm production on six different surfaces with or without the addition of serum: polycarbonate, polystyrene, stainless steel, Teflon, polyvinyl chloride or hydroxyapatite. Our results showed that serum increases in vitro C. albicans biofilm formation on a wide range of distinct surfaces including metallic and non-metallic materials, and that roughness and hydrophobicity can modulate C. albicans biofilm formation. These findings were also confirmed by scanning electron microscopy and it revealed the deposition of extracellular material on hyphae attached to a solid surface. Interestingly, adhesion can be significantly increased in the early stages of colonisation when serum is provided as a conditioning film in a surface-dependent manner.
念珠菌生物膜可以在多种材料上形成，包括植入的医疗器械，并对抗真菌药物表现出耐药表型。包括宿主和表面特性在内的多种因素可能会影响生物体内和生物体外的白色念珠菌生物膜的建立和发展。因此，我们选择了一组白色念珠菌临床分离株，以评估表面和血清对生物膜附着和发展的影响。使用 CDC 生物膜反应器中的圆盘从培养皿中检测在有无血清添加的情况下，六种不同表面（聚碳酸酯、聚苯乙烯、不锈钢、特氟龙、聚氯乙烯或羟基磷灰石）上的生物膜产生情况。我们的结果表明，血清可增加体外多种不同表面（包括金属和非金属材料）上白色念珠菌生物膜的形成，而粗糙度和疏水性可以调节白色念珠菌生物膜的形成。扫描电子显微镜也证实了这一点，它显示了附着在固体表面上的菌丝上的细胞外物质的沉积。有趣的是，当血清以表面依赖的方式作为条件膜提供时，在定植的早期阶段，粘附可以显著增加。
