Centre for Biotechnology, Anna University, Chennai, India.
Clin Chim Acta. 2011 Sep 18;412(19-20):1867-73. doi: 10.1016/j.cca.2011.06.029. Epub 2011 Jun 30.
Lymphatic filariasis is a painful and profoundly disfiguring disease. Infection is usually acquired in childhood but its visible manifestations occur later in life, causing temporary or permanent disability. The importance of developing effective assays to diagnose, monitor and evaluate human lymphatic filariasis has been emphasized by the WHO.
High-affinity monoclonal antibodies (mAbs) specific for recombinant filarial antigen WbSXP-1 were developed. An ELISA based capture assay using monoclonal and polyclonal antibodies for WbSXP-1 was used for detection of circulating filarial antigen.
High-affinity monoclonal antibodies (mAbs) were developed that specifically binds both W. bancrofti and B. malayi mf antigens. Two mAbs (1F6H3 and 2E12E3) of subclass IgG2a and IgM showed high affinity, avidity and reactivity to recombinant and mf native antigen. Both the mAbs were used in combination as capture antibodies and polyclonal as detection antibody to develop the assay. The assay showed very high sensitivity towards W. bancrofti mf positive samples compared to endemic normal samples (P<0.0001).
A capture assay using high-affinity monoclonal antibodies for WbSXP-1 was developed for the detection of filarial circulating antigen in clinical samples from bancroftian infection. Besides, this would also help in epidemiological studies in endemic areas of filarial infections.
淋巴丝虫病是一种痛苦且严重毁容的疾病。感染通常发生在儿童时期，但它的可见症状出现在生命后期，导致暂时或永久性残疾。世界卫生组织强调了开发有效检测方法来诊断、监测和评估人体淋巴丝虫病的重要性。
开发了针对重组丝虫抗原 WbSXP-1 的高亲和力单克隆抗体 (mAb)。使用单克隆和多克隆抗体针对 WbSXP-1 的 ELISA 捕获检测用于检测循环丝虫抗原。
开发了高亲和力的单克隆抗体 (mAb)，可特异性结合 W. bancrofti 和 B. malayi mf 抗原。两种亚类 IgG2a 和 IgM 的 mAb（1F6H3 和 2E12E3）具有高亲和力、亲合力和对重组和 mf 天然抗原的反应性。两种 mAb 均被组合用作捕获抗体，多克隆抗体用作检测抗体来开发该检测方法。与流行地区的正常样本相比，该检测方法对 W. bancrofti mf 阳性样本具有非常高的敏感性 (P<0.0001)。
针对 WbSXP-1 开发了使用高亲和力单克隆抗体的捕获检测方法，用于检测班氏丝虫感染的临床样本中的丝虫循环抗原。此外，这还有助于在丝虫感染流行地区进行流行病学研究。
