Department of Agricultural Sciences, School of Life Sciences, La Trobe University 3086, Australia.
J Dairy Sci. 2010 Apr;93(4):1355-63. doi: 10.3168/jds.2009-2395.
Many bacteria respond to potentially growth-limiting availability of iron by producing low-molecular-weight iron chelators (siderophores). The aim of this work was to examine the siderophores synthesized and utilized by Pseudomonas spp. implicated in milk spoilage. Twenty isolates of Pseudomonas spp. previously shown to have significant milk spoilage potential were tested for the ability to produce siderophores. Of these, 14 produced pyoverdin and 2 of these also produced pyochelin; 1 produced only pyochelin; 1 produced only salicylate; 2 produced non-pyoverdin, hydroxamate-containing siderophore; and 2 produced chrome azurol sulfonate reactive material that was neither pyoverdin nor pyochelin. There was considerable diversity among the pyoverdins produced. All isolates were shown to utilize iron complexed with exogenous pyoverdin, but usage of particular exogenous pyoverdins differed among isolates. Interference with the iron-uptake systems of the Pseudomonas spp. may be a means by which food spoilage can be slowed, and the pyoverdin system would appear to be a potential target. However, given the diversity of pyoverdins produced and utilized, and the presence of other siderophores, successful interference with bacterial iron acquisition in this context may be challenging.
许多细菌通过产生低分子量的铁螯合剂（ siderophores ）来应对潜在的生长限制的铁可用性。本工作的目的是研究与牛奶腐败有关的假单胞菌合成和利用的 siderophores 。先前显示具有显著牛奶腐败潜力的 20 株假单胞菌分离株被测试产生 siderophores 的能力。其中，14 株产生了绿脓菌素，其中 2 株也产生了 pyochelin ； 1 株只产生了 pyochelin ； 1 株只产生了水杨酸； 2 株产生了非绿脓菌素、含有羟肟酸盐的 siderophore ； 2 株产生了铬天青 S 反应性物质，既不是绿脓菌素也不是 pyochelin 。所产生的绿脓菌素之间存在相当大的多样性。所有分离株都被证明能够利用与外源绿脓菌素络合的铁，但特定外源绿脓菌素的使用在分离株之间存在差异。干扰假单胞菌的铁摄取系统可能是减缓食物腐败的一种方法，而绿脓菌素系统似乎是一个潜在的目标。然而，考虑到所产生和利用的绿脓菌素的多样性，以及其他 siderophores 的存在，在这种情况下成功干扰细菌铁的摄取可能具有挑战性。
