肠杆菌属肠道外分离株通过铁载体介导的铁获取策略

Siderophore-mediated strategies of iron acquisition by extraintestinal isolates of Enterobacter spp.

作者信息

Mokracka Joanna, Kaznowski Adam, Szarata Małgorzata, Kaczmarek Ewa

机构信息

Department of Microbiology, Institute of Experimental Biology, A. Mickiewicz University, Fredry 10, 61-701 Poznań, Poland.

出版信息

Acta Microbiol Pol. 2003;52(1):81-6.

PMID:12916730

Abstract

A total of 89 examined Enterobacter isolates belonging to three species: E. cloaceae, E. aerogenes and E. sakazakii, produced iron chelators detected in universal CAS assay. In chemical assays the strains were shown to excrete mostly catecholate (88 strains) and hydroxamate (42 strains) type of siderophores. Forty-one strains produced both catecholate and hydroxamate siderophores whereas one isolate produced only hydroxamate. Besides, the isolates were screened for genes coding for another siderophore: yersiniabactin. The genes for biosynthesis and uptake of yersiniabactin are located on the high-pathogenicity island (HPI) of Yersinia spp. The presence of three marker genes irp1, irp2 and fyuA was estimated by polymerase chain reaction. Two strains: E. aerogenes and E. cloaceae possessed irp1, irp2 and fyuA genes. PCR products of irp1, irp2 and fyuA were of 240, 280 and 780 bp, respectively.

摘要

总共89株经检测的肠杆菌分离株，分属于三种菌：阴沟肠杆菌、产气肠杆菌和阪崎肠杆菌，在通用CAS试验中检测到它们产生铁螯合剂。在化学分析中，这些菌株大多分泌儿茶酚型（88株）和异羟肟酸型（42株）铁载体。41株菌株同时产生儿茶酚型和异羟肟酸型铁载体，而1株分离株仅产生异羟肟酸型铁载体。此外，对这些分离株进行筛选，检测编码另一种铁载体——耶尔森菌素的基因。耶尔森菌素的生物合成和摄取基因位于耶尔森菌属的高致病性岛上。通过聚合酶链反应评估三个标记基因irp1、irp2和fyuA的存在情况。两株菌：产气肠杆菌和阴沟肠杆菌拥有irp1、irp2和fyuA基因。irp1、irp2和fyuA的PCR产物分别为240 bp、280 bp和780 bp。