Baslé Emmanuel, Joubert Nicolas, Pucheault Mathieu
Molecular Chemistry and Photonic, UMR 6510 CPM, Centre National de la Recherche Scientifique, Université de Rennes1, 263 Avenue du Général Leclerc, 35042 Rennes cedex, France.
Chem Biol. 2010 Mar 26;17(3):213-27. doi: 10.1016/j.chembiol.2010.02.008.
Chemical modification of protein is an arduous but fruitful task. Many chemical methods have been developed for such purpose by carefully balancing reactivity and selectivity. Now both chemists and biologists have in hand an arsenal of tools from which they can select a relevant reaction to tackle their problems. This review focuses on the various chemical transformations available for selective modification of proteins. It also provides a brief overview of some of their main applications, including detection of protein interactions, preparation of bioconjugates, and protein microarrays.
蛋白质的化学修饰是一项艰巨但富有成果的任务。为了实现这一目标，人们通过仔细平衡反应活性和选择性，开发了许多化学方法。现在，化学家和生物学家都拥有大量的工具库，从中可以选择相关反应来解决他们的问题。本综述重点介绍了可用于蛋白质选择性修饰的各种化学转化。它还简要概述了它们的一些主要应用，包括蛋白质相互作用的检测、生物共轭物的制备以及蛋白质微阵列。
