Università degli Studi di Milano, Dipartimento di Scienze Farmacologiche, via Balzaretti 9, 20133 Milano, Italy.
J Cyst Fibros. 2010 May;9(3):228-33. doi: 10.1016/j.jcf.2010.03.002. Epub 2010 Mar 24.
To assess fatty acid (FA) profiles in whole blood of 90 cystic fibrosis patients (CF) and 30 control subjects (C) and to correlate FA changes to the severity of respiratory disease.
Whole blood FA were assessed by GC with a micromethod-based analysis.
Saturated and monounsaturated FA are higher, whereas polyunsaturated FA are lower in CF versus C with reduction of total n-6 FA, 22:5n-3 and 22:6n-3 (DHA). The product of linoleic acid (LA) x DHA, proposed as a marker for the disease, is 30% lower in CF than in C. Correlations with the severity of the respiratory disease are present for different FA and for the LA x DHA product. There is a reduction of Delta5 desaturase activity in CF, greater in severe disease, suggesting a basic metabolic alteration.
The micromethod-based analysis of blood FA facilitates the assessment of the FA status while confirming alterations of FA profiles already reported in specific blood compartments of CF.
评估 90 例囊性纤维化（CF）患者和 30 例对照者全血中的脂肪酸（FA）谱，并将 FA 变化与呼吸道疾病的严重程度相关联。
采用基于微方法的 GC 分析评估全血 FA。
与对照者相比，CF 患者的饱和和单不饱和 FA 更高，而多不饱和 FA 更低，总 n-6 FA、22:5n-3 和 22:6n-3（DHA）减少。作为疾病标志物的亚油酸（LA）x DHA 的产物在 CF 中比对照者低 30%。不同 FA 和 LA x DHA 产物与呼吸道疾病的严重程度相关。CF 中存在 Delta5 去饱和酶活性降低，在严重疾病中更为明显，提示存在基本代谢改变。
基于微方法的血液 FA 分析有助于评估 FA 状态，同时证实 CF 特定血液成分中已报道的 FA 谱改变。
