School of Life Sciences and Biotechnology, Korea University, Seoul 136-701, Korea.
J Radiat Res. 2010;51(2):205-13. doi: 10.1269/jrr.09030.
Although many in vitro studies have previously been conducted to elucidate the biological effects of radio frequency (RF) radiation over the past decades, the existence and nature of any effects is still inconclusive. In an effort to further elucidate this question, we have monitored changes in protein expression profiles in RF-exposed MCF7 human breast cancer cells using two-dimensional gel electrophoresis. MCF7 cells were exposed to 849 MHz RF radiation for 1 h per day for three consecutive days at specific absorption rates (SARs) of either 2 W/Kg or 10 W/kg. During exposure, the temperature in the exposure chamber was kept in an isothermal condition. Twenty-four hours after the final RF exposure, the protein lysates from MCF cells were prepared and two-dimensional electrophoretic analyses were conducted. The protein expression profiles of the MCF cells were not significantly altered as the result of RF exposure. None of the protein spots on the two-dimensional electrophoretic gels showed reproducible changes in three independent experiments. To determine effect of RF radiation on protein expression profiles more clearly, three spots showing altered expression without reproducibility were identified using electrospray ionization tandem mass spectrometry analysis and their expressions were examined with RT-PCR and Western blot assays. There was no alteration in their mRNA and protein levels. As we were unable to observe any significant and reproducible changes in the protein expression profiles of the RF radiation-exposed MCF7 cells using high throughput and non-high throughput techniques, it seems unlikely that RF exposure modulates the protein expression profile.
尽管在过去的几十年里已经进行了许多体外研究来阐明射频 (RF) 辐射的生物学效应，但任何效应的存在和性质仍然不确定。为了进一步阐明这个问题，我们使用二维凝胶电泳监测了暴露于 RF 的 MCF7 人乳腺癌细胞中蛋白质表达谱的变化。MCF7 细胞每天在特定吸收率 (SAR) 为 2 W/Kg 或 10 W/kg 的情况下暴露于 849 MHz RF 辐射 1 小时，连续暴露 3 天。在暴露过程中，暴露室内的温度保持等温状态。在最后一次 RF 暴露后 24 小时，从 MCF 细胞中提取蛋白质裂解物并进行二维电泳分析。RF 暴露并未导致 MCF 细胞的蛋白质表达谱发生明显改变。在三个独立的实验中，二维电泳凝胶上没有一个蛋白质斑点显示出可重复的变化。为了更清楚地确定 RF 辐射对蛋白质表达谱的影响，使用电喷雾电离串联质谱分析鉴定了三个表达改变但无重复性的斑点，并使用 RT-PCR 和 Western blot 分析检查了它们的表达。它们的 mRNA 和蛋白质水平没有改变。由于我们无法使用高通量和非高通量技术观察到 RF 辐射暴露的 MCF7 细胞蛋白质表达谱的任何显著和可重复的变化，因此 RF 暴露似乎不太可能调节蛋白质表达谱。
