STUK - Radiation and Nuclear Safety Authority, Helsinki, Finland.
Proteome Sci. 2010 Oct 18;8:52. doi: 10.1186/1477-5956-8-52.
Use of mobile phones has widely increased over the past decade. However, in spite of the extensive research, the question of potential health effects of the mobile phone radiation remains unanswered. We have earlier proposed, and applied, proteomics as a tool to study biological effects of the mobile phone radiation, using as a model human endothelial cell line EA.hy926. Exposure of EA.hy926 cells to 900 MHz GSM radiation has caused statistically significant changes in expression of numerous proteins. However, exposure of EA.hy926 cells to 1800 MHz GSM signal had only very small effect on cell proteome, as compared with 900 MHz GSM exposure. In the present study, using as model human primary endothelial cells, we have examined whether exposure to 1800 MHz GSM mobile phone radiation can affect cell proteome.
Primary human umbilical vein endothelial cells and primary human brain microvascular endothelial cells were exposed for 1 hour to 1800 MHz GSM mobile phone radiation at an average specific absorption rate of 2.0 W/kg. The cells were harvested immediately after the exposure and the protein expression patterns of the sham-exposed and radiation-exposed cells were examined using two dimensional difference gel electrophoresis-based proteomics (2DE-DIGE). There were observed numerous differences between the proteomes of human umbilical vein endothelial cells and human brain microvascular endothelial cells (both sham-exposed). These differences are most likely representing physiological differences between endothelia in different vascular beds. However, the exposure of both types of primary endothelial cells to mobile phone radiation did not cause any statistically significant changes in protein expression.
Exposure of primary human endothelial cells to the mobile phone radiation, 1800 MHz GSM signal for 1 hour at an average specific absorption rate of 2.0 W/kg, does not affect protein expression, when the proteomes were examined immediately after the end of the exposure and when the false discovery rate correction was applied to analysis. This observation agrees with our earlier study showing that the 1800 MHz GSM radiation exposure had only very limited effect on the proteome of human endothelial cell line EA.hy926, as compared with the effect of 900 MHz GSM radiation.
在过去的十年中，移动电话的使用广泛增加。然而，尽管进行了广泛的研究，手机辐射的潜在健康影响问题仍未得到解答。我们之前提出并应用蛋白质组学作为研究手机辐射生物学效应的工具，使用人内皮细胞系 EA.hy926 作为模型。暴露于 900MHz GSM 辐射的 EA.hy926 细胞的表达发生了统计学上显著的变化。然而，与 900MHz GSM 辐射暴露相比，1800MHz GSM 信号对 EA.hy926 细胞的细胞蛋白质组仅有非常小的影响。在本研究中，使用人原代内皮细胞作为模型，我们检查了 1800MHz GSM 手机辐射是否会影响细胞蛋白质组。
将原代人脐静脉内皮细胞和原代人脑微血管内皮细胞暴露于 1800MHz GSM 手机辐射下 1 小时，平均比吸收率为 2.0W/kg。暴露后立即收获细胞，并使用二维差异凝胶电泳蛋白质组学（2DE-DIGE）检查假暴露和辐射暴露细胞的蛋白质表达模式。观察到人脐静脉内皮细胞和人脑微血管内皮细胞的蛋白质组之间存在许多差异（均为假暴露）。这些差异很可能代表不同血管床内皮之间的生理差异。然而，两种类型的原代内皮细胞暴露于手机辐射并未导致蛋白质表达的任何统计学上显著变化。
将原代人内皮细胞暴露于手机辐射下，1800MHz GSM 信号 1 小时，平均比吸收率为 2.0W/kg，当暴露结束后立即检查蛋白质组，并对分析应用错误发现率校正时，不会影响蛋白质表达。这一观察结果与我们之前的研究一致，即与 900MHz GSM 辐射的影响相比，1800MHz GSM 辐射对人内皮细胞系 EA.hy926 的蛋白质组仅有非常有限的影响。
