Alsheimer M, Baier A, Schramm S, Schütz W, Benavente R
Department of Cell and Developmental Biology, Biocenter, University of Würzburg, DE-97074 Würzburg, Germany.
Cytogenet Genome Res. 2010;128(1-3):162-8. doi: 10.1159/000303341. Epub 2010 Mar 25.
Meiosis-specific protein SYCP3 is a major structural component of synaptonemal complex (SC) lateral elements. SYCP3 is rather well conserved in vertebrates. However, some differences in SYCP3 expression have been shown among mammals. To clarifiy these differences, we have investigated SYCP3 expression with the aid of bioinformatic, biochemical and cell biological methods. Here we show that, in contrast to other vertebrates, rat and mouse SYCP3 exist in 2 isoforms. The short isoform is conserved among vertebrates. However, the longer isoform, which represents an N-terminal extension of the shorter one, most likely appeared about 15 million years ago in a common ancestor of rat and mouse and after separation from the hamster branch.
减数分裂特异性蛋白SYCP3是联会复合体（SC）侧生元件的主要结构成分。SYCP3在脊椎动物中相当保守。然而，已显示哺乳动物之间SYCP3表达存在一些差异。为了阐明这些差异，我们借助生物信息学、生物化学和细胞生物学方法研究了SYCP3的表达。在此我们表明，与其他脊椎动物不同，大鼠和小鼠的SYCP3存在两种异构体。短异构体在脊椎动物中是保守的。然而，较长的异构体是较短异构体的N端延伸，很可能在约1500万年前出现在大鼠和小鼠的共同祖先中，并在与仓鼠分支分离之后。
