Department of Orthopedics, the Third Affiliated Hospital of Sun Yat-sen University, Tian He District, No. 600 Tianhe Road, Guangzhou, Guangdong, P.R. China.
J Endocrinol Invest. 2010 Oct;33(9):644-9. doi: 10.1007/BF03346664. Epub 2010 Mar 25.
The purpose of this study was to investigate whether serum levels of selected endogenous estrogens and their metabolites are involved in the pathogenesis of osteoarthritis in pre- and post-menopausal women with osteoarthritis. Sixty-four patients with osteoarthritis (OA) of the knee, 48 patients with rheumatoid arthritis (RA) of the knee, and 48 healthy women were included in this study. Serum concentrations of estradiol and estrogen metabolites, such as 2- hydroxyestrone, 2-hydroxyestradiol, and 16α-hydroxyestrone, were measured by high performance liquid chromatography-mass spectrometry. Our results show that the serum concentrations of free estradiol and total 2-hydroxyestrone were significantly lower in pre-menopausal women with OA compared to the levels detected in the control groups (RA and healthy women). While serum concentrations of free and total estradiol in post-menopausal women with OA was significantly decreased compared to those of the control groups, the level of total 2-hydroxyestradiol significantly increased in postmenopausal women. Furthermore, the total 2-hydroxyestrone concentration positively correlated with the total estradiol level in pre-menopausal women with OA. In addition, the total 2- hydroxyestradiol level positively correlated with free and total estradiol levels in post-menopausal women with OA. In conclusion, estradiol and estrogen metabolites, including 2-hydroxyestrone and 2-hydroxyestradiol, were found in the sera of pre- and post-menopausal women with OA. Except for free and total estradiol deficiency, a decreased serum level of total 2- hydroxyestrone in pre-menopausal women and an increased total 2-hydroxyestradiol level in post-menopausal women with OA may also correlate with the pathogenesis of female OA.
本研究旨在探讨绝经前和绝经后女性骨关节炎患者的血清中选定的内源性雌激素及其代谢物水平是否与骨关节炎的发病机制有关。本研究纳入了 64 例膝关节骨关节炎（OA）患者、48 例膝关节类风湿关节炎（RA）患者和 48 例健康女性。采用高效液相色谱-质谱法测定雌二醇和雌激素代谢物（如 2-羟雌酮、2-羟雌二醇和 16α-羟雌酮）的血清浓度。我们的研究结果表明，与对照组（RA 和健康女性）相比，绝经前 OA 患者的血清游离雌二醇和总 2-羟雌酮浓度显著降低。而绝经后 OA 患者的血清游离和总雌二醇水平明显低于对照组，总 2-羟雌二醇水平明显升高。此外，绝经前 OA 患者的总 2-羟雌酮浓度与总雌二醇水平呈正相关。另外，绝经后 OA 患者的总 2-羟雌二醇水平与游离和总雌二醇水平呈正相关。总之，在绝经前和绝经后 OA 女性的血清中发现了雌二醇和雌激素代谢物，包括 2-羟雌酮和 2-羟雌二醇。除了游离和总雌二醇缺乏外，绝经前 OA 女性的总 2-羟雌酮血清水平降低和绝经后 OA 女性的总 2-羟雌二醇水平升高可能也与女性 OA 的发病机制有关。
