绝经前和绝经后妇女以及患有甲状腺乳头癌的男性的尿甾体代谢改变。
Metabolic alteration of urinary steroids in pre- and post-menopausal women, and men with papillary thyroid carcinoma.
机构信息
Future Convergence Research Division, Korea Institute of Science and Technology, Seoul 136-791, Korea.
出版信息
BMC Cancer. 2011 Aug 8;11:342. doi: 10.1186/1471-2407-11-342.
BACKGROUND
To evaluate the metabolic changes in urinary steroids in pre- and post-menopausal women and men with papillary thyroid carcinoma (PTC).
METHODS
Quantitative steroid profiling combined with gas chromatography-mass spectrometry was used to measure the urinary concentrations of 84 steroids in both pre- (n = 21, age: 36.95 ± 7.19 yr) and post-menopausal female (n = 19, age: 52.79 ± 7.66 yr), and male (n = 16, age: 41.88 ± 8.48 yr) patients with PTC. After comparing the quantitative data of the patients with their corresponding controls (pre-menopause women: n = 24, age: 33.21 ± 10.48 yr, post-menopause women: n = 16, age: 49.67 ± 8.94 yr, male: n = 20, age: 42.75 ± 4.22 yr), the levels of steroids in the patients were normalized to the mean concentration of the controls to exclude gender and menopausal variations.
RESULTS
Many urinary steroids were up-regulated in all PTC patients compared to the controls. Among them, the levels of three active androgens, androstenedione, androstenediol and 16α-hydroxy DHEA, were significantly higher in the pre-menopausal women and men with PTC. The corticoid levels were increased slightly in the PTC men, while progestins were not altered in the post-menopausal PTC women. Estrogens were up-regulated in all PTC patients but 2-hydroxyestrone and 2-hydroxy-17β-estradiol were remarkably changed in both pre-menopausal women and men with PTC. For both menopausal and gender differences, the 2-hydroxylation, 4-hydroxylation, 2-methoxylation, and 4-methoxylation of estrogens and 16α-hydroxylation of DHEA were differentiated between pre- and post-menopausal PTC women (P < 0.001). In particular, the metabolic ratio of 2-hydroxyestrone to 2-hydroxy-17β-estradiol, which could reveal the enzyme activity of 17β-hydroxysteroid dehydrogenase, showed gender differences in PTC patients (P < 1 × 10-7).
CONCLUSIONS
These results are expected be helpful for better understanding the pathogenic differences in PTC according to gender and menopausal conditions.
背景
评估绝经前、后妇女和男性甲状腺乳头状癌（PTC）患者尿甾体代谢变化。
方法
采用定量类固醇谱分析联合气相色谱-质谱法测定 21 例绝经前（年龄：36.95 ± 7.19 岁）、19 例绝经后（年龄：52.79 ± 7.66 岁）和 16 例男性（年龄：41.88 ± 8.48 岁）PTC 患者的尿中 84 种甾体浓度。将患者的定量数据与相应的对照组（绝经前女性：n = 24，年龄：33.21 ± 10.48 岁；绝经后女性：n = 16，年龄：49.67 ± 8.94 岁；男性：n = 20，年龄：42.75 ± 4.22 岁）进行比较，将患者的甾体水平标准化为对照组的平均浓度，以排除性别和绝经差异。
结果
与对照组相比，所有 PTC 患者的许多尿甾体均升高。其中，绝经前妇女和男性 PTC 患者的三种活性雄激素（雄烯二酮、雄烯二醇和 16α-羟基 DHEA）水平明显升高。PTC 男性的皮质醇水平略有升高，而绝经后 PTC 女性的孕激素没有改变。所有 PTC 患者的雌激素均升高，但绝经前妇女和男性的 2-羟基雌酮和 2-羟基-17β-雌二醇变化显著。对于绝经前和绝经后以及性别差异，PTC 绝经前和绝经后女性的雌激素的 2-羟化、4-羟化、2-甲氧基化和 4-甲氧基化以及 DHEA 的 16α-羟化均有差异（P < 0.001）。特别是，2-羟基雌酮与 2-羟基-17β-雌二醇的代谢比值可以揭示 17β-羟甾脱氢酶的酶活性，在 PTC 患者中存在性别差异（P < 1 × 10-7）。
结论
这些结果有望有助于更好地根据性别和绝经状况理解 PTC 的发病差异。
Zotero 插件