Department of Surgery, Kitasato University School of Medicine, Sagamihara, Japan.
Ann Surg Oncol. 2010 Sep;17(9):2341-8. doi: 10.1245/s10434-010-1002-7. Epub 2010 Mar 26.
Lymph node metastasis is widely accepted as one of the most important determinants of prognosis in colorectal cancer (CRC) patients. Therefore, there is an urgent need to identify molecular markers that can be used to predict lymph node metastasis.
Candidate genes were found using LMD and cDNA microarrays in a large-scale study of CRC, followed by Penalized Canonical Correlation Analysis (PCCA). We focused on the Fanconi anemia, complementation group D2 (FANCD2) gene and evaluated FANCD2 mRNA expression in 133 CRC cases to determine the clinicopathological significance of FANCD2 expression.
The mean level of FANCD2 mRNA expression in tumor tissue specimens was significantly higher than in nontumor tissue. FANCD2 expression was found to be a significant factor affecting lymph node metastasis: the high FANCD2 expression group had a significantly poorer prognosis than the low expression group.
This study suggests that PCCA can be used to identify genes related to clinicopathological features. Furthermore, high FANCD2 expression was a significant independent factor for lymph node metastasis.
淋巴结转移被广泛认为是结直肠癌（CRC）患者预后的最重要决定因素之一。因此，迫切需要确定可用于预测淋巴结转移的分子标志物。
在 CRC 的大规模研究中，使用 LMD 和 cDNA 微阵列找到候选基因，然后进行惩罚典型相关分析（PCCA）。我们专注于范可尼贫血症，补体组 D2（FANCD2）基因，并评估 133 例 CRC 病例中的 FANCD2 mRNA 表达，以确定 FANCD2 表达的临床病理意义。
肿瘤组织标本中 FANCD2 mRNA 表达的平均水平明显高于非肿瘤组织。FANCD2 表达是影响淋巴结转移的显著因素：高 FANCD2 表达组的预后明显比低表达组差。
这项研究表明，PCCA 可用于鉴定与临床病理特征相关的基因。此外，高 FANCD2 表达是淋巴结转移的显著独立因素。
