Kinoshita Hisoka, Hori Yuichi, Fukumoto Takumi, Ohigashi Takuji, Shinohara Kunio, Hayashi Yoshitake, Ku Yonson
Department of Surgery, Division of Hepato-Biliary-Pancreatic Surgery, Kobe University Graduate School of Medicine, Kobe, 650-0017, Japan.
Med Mol Morphol. 2010 Mar;43(1):19-25. doi: 10.1007/s00795-009-0474-7. Epub 2010 Mar 26.
Excess iron deposition in the liver is known to be hepatotoxic and may exacerbate liver injury. However, little is known about iron distribution in the lobule because of the lack of a highly sensitive detection method. The aim of this study is to determine iron distribution in the lobule of human liver by means of synchrotron radiation X-ray fluorescence (SRXRF) microscopy. Mapping of the trace elements was done with use of SRXRF microscopy and compared with the results of staining by Berlin blue and oxidative stress markers. Iron deposits were distributed predominantly in periportal hepatocytes in the normal liver in a decreasing gradient from the periportal area to the perivenous area. This distribution was consistent with the formation of oxidative stress markers, suggesting that hepatocytes in the periportal area may be predominantly primed by iron-induced free radical damage even in normal liver. On the other hand, iron deposits in the periportal area were more intense than those in the centrilobular area in both the liver with chronic hepatitis C and the cirrhotic liver. In conclusion, elemental mapping by SRXRF microscopy was a highly sensitive method for the detection and mapping of elements such as iron and copper in liver sections.
肝脏中铁过量沉积具有肝毒性，可能会加重肝损伤。然而，由于缺乏高灵敏度的检测方法，关于肝小叶中铁的分布情况鲜为人知。本研究的目的是通过同步辐射X射线荧光（SRXRF）显微镜确定人肝小叶中铁的分布。利用SRXRF显微镜对微量元素进行成像，并与普鲁士蓝染色结果和氧化应激标志物进行比较。正常肝脏中铁沉积主要分布在汇管区周围的肝细胞中，从汇管区到中央静脉区呈递减梯度。这种分布与氧化应激标志物的形成一致，表明即使在正常肝脏中，汇管区周围的肝细胞可能主要受到铁诱导的自由基损伤。另一方面，在丙型肝炎慢性期肝脏和肝硬化肝脏中，汇管区的铁沉积均比小叶中央区更为明显。总之，SRXRF显微镜元素成像对于检测和定位肝脏切片中的铁和铜等元素是一种高灵敏度的方法。
