Walter Reed Army Institute of Research, Division of Psychiatry and Neuroscience, 503 Robert Grant Avenue, Silver Spring, MD 20910, USA.
J Neurosci Methods. 2010 May 30;189(1):84-7. doi: 10.1016/j.jneumeth.2010.03.012. Epub 2010 Mar 25.
A rat model of penetrating ballistic-like brain injury (PBBI) was recently established to study military-relevant severe traumatic brain injury (TBI). The purpose of this study was to conduct a side-by-side evaluation of two well-established cognitive testing paradigms: the novel object recognition (NOR) task and the Morris water maze (MWM) task. Accordingly, male Sprague-Dawley rats were subjected to PBBI and their cognitive abilities were assessed at 7 and 21 days post-PBBI. Although PBBI animals had more difficulty completing both tasks compared to sham animals, their performance on the NOR task was confounded by a high degree of within-group variability that was likely due to attention deficits produced by the injury. In contrast, PBBI produced consistent, significant spatial learning deficits in the MWM task. Overall, these results suggest that the MWM task provides a more appropriate cognitive test for the PBBI model that would be useful for testing promising neuroprotective therapeutics.
最近建立了一种穿透性弹道样脑损伤（PBBI）大鼠模型，用于研究与军事相关的严重创伤性脑损伤（TBI）。本研究的目的是对两种成熟的认知测试范式进行并列评估：新物体识别（NOR）任务和 Morris 水迷宫（MWM）任务。因此，雄性 Sprague-Dawley 大鼠接受 PBBI 后，在 PBBI 后 7 天和 21 天评估其认知能力。尽管 PBBI 动物在完成这两项任务时比假手术动物更困难，但它们在 NOR 任务中的表现受到了由于损伤引起的注意力缺陷导致的高组内变异性的混淆。相比之下，PBBI 在 MWM 任务中产生了一致的、显著的空间学习缺陷。总的来说，这些结果表明 MWM 任务为 PBBI 模型提供了一种更合适的认知测试方法，对于测试有前途的神经保护治疗方法非常有用。
