Okano Wataru, Nomoto Yukio, Wada Ikuo, Kobayashi Ken, Miyake Masao, Nakamura Tatsuo, Omori Koichi
Department of Otolaryngology, School of Medicine, Fukushima Medical University, Fukushima City, Japan.
Ann Otol Rhinol Laryngol. 2009 Nov;118(11):796-804.
Although our group has had mostly successful results with clinical application of a tracheal prosthesis, delayed epithelial regeneration remains a problem. In our previous studies using rats, it was demonstrated that tracheal fibroblasts accelerated proliferation and differentiation of the tracheal epithelium in vitro and in vivo. The purpose of this study was to evaluate the effects of fibroblasts on epithelial regeneration in larger tracheal defects in rabbits.
We developed a bioengineered scaffold, the luminal surface of which was coated with fibroblasts. This scaffold was implanted into tracheal defects in 12 rabbits (bioengineered group), and scaffolds without fibroblasts were implanted in 12 rabbits (control group). The regenerated epithelium was histologically examined by light microscopy, scanning electron microscopy, and immunohistochemical studies.
In the bioengineered group, a stratified squamous epithelium was observed on the surface 7 days after transplantation. However, in the control group, the scaffolds were exposed. Fourteen days after implantation, a columnar ciliated epithelium was observed in the bioengineered group. The average thickness of the regenerated epithelium in the bioengineered group was significantly greater than that in the control group.
This study indicated that fibroblasts had a stimulatory effect that hastened regeneration of the epithelium in large tracheal defects.
尽管我们团队在气管假体的临床应用方面大多取得了成功的结果，但上皮再生延迟仍然是一个问题。在我们之前使用大鼠进行的研究中，已证明气管成纤维细胞在体外和体内均可加速气管上皮细胞的增殖和分化。本研究的目的是评估成纤维细胞对兔较大气管缺损上皮再生的影响。
我们开发了一种生物工程支架，其管腔表面涂有成纤维细胞。将该支架植入12只兔的气管缺损处（生物工程组），并将未涂有成纤维细胞的支架植入12只兔体内（对照组）。通过光学显微镜、扫描电子显微镜和免疫组织化学研究对再生上皮进行组织学检查。
在生物工程组中，移植后7天在表面观察到复层鳞状上皮。然而，在对照组中，支架暴露。植入后14天，生物工程组观察到柱状纤毛上皮。生物工程组再生上皮的平均厚度明显大于对照组。
本研究表明，成纤维细胞具有促进作用，可加速大气管缺损处上皮的再生。
