Department of Pathology, Singapore General Hospital, Outram Road, Singapore 169608, Singapore.
Breast. 2010 Oct;19(5):355-9. doi: 10.1016/j.breast.2010.02.007. Epub 2010 Mar 26.
There are limited data that compare the utility of immunohistochemical detection of mammaglobin with Gross Cystic Disease Fluid Protein-15 (GCDFP-15) in locally recurrent and metastatic breast cancers. Forty-three local and 72 distant recurrences of breast cancer, 8 metastatic lesions to the breast from other organs, and 30 metastases from non-breast primaries were immunohistochemically stained with mammaglobin and GCDFP-15 antibodies. Mammaglobin was expressed in 55 (47.8%) and GCDFP-15 detected in 13 (11.3%) locally and distantly recurrent breast cancers. A higher percentage of tumor cells was stained with mammaglobin at greater staining intensity than GCDFP-15, for both metastatic and locally recurrent breast cancers. The difference in staining intensity as well as mean percentage of tumor cells stained for both markers was statistically significant (p < 0.005). Metastases to the breast from other organs and metastatic lesions from non-breast primaries were uniformly negative for both mammaglobin and GCDFP-15. Our study demonstrates that immunohistochemical analysis of mammaglobin is superior to GCDFP-15 in detecting a tumor of breast origin, and can be incorporated into immunohistochemical panels evaluating tumors from unknown primary sites.
在局部复发性和转移性乳腺癌中，比较免疫组化检测乳球蛋白与 Gross Cystic Disease Fluid Protein-15（GCDFP-15）的效用的数据有限。我们对 43 例局部复发和 72 例远处转移的乳腺癌、8 例来自其他器官的转移性乳腺癌和 30 例来自非乳腺原发灶的转移性乳腺癌进行了免疫组化染色，分别使用乳球蛋白和 GCDFP-15 抗体。在局部和远处复发性乳腺癌中，有 55 例（47.8%）表达乳球蛋白，13 例（11.3%）检测到 GCDFP-15。对于转移性和局部复发性乳腺癌，用乳球蛋白染色的肿瘤细胞百分比和染色强度均高于 GCDFP-15。两种标志物的染色强度差异以及肿瘤细胞的平均染色百分比均具有统计学意义（p<0.005）。来自其他器官的转移性乳腺癌和来自非乳腺原发灶的转移性病变对乳球蛋白和 GCDFP-15 均呈阴性。我们的研究表明，免疫组化分析乳球蛋白在检测乳腺来源的肿瘤方面优于 GCDFP-15，可纳入评估不明原发灶肿瘤的免疫组化分析。
