Shield Paul W, Crouch Stephen J, Papadimos David J, Walsh Michael D
School of Biomedical Sciences, Queensland University of Technology, Brisbane, Queensland, Australia.
Department of Cytology, Sullivan Nicolaides Pathology, Bowen Hills, Queensland, Australia.
J Cytol. 2018 Apr-Jun;35(2):90-93. doi: 10.4103/JOC.JOC_132_17.
The utility of GATA3 immunohistochemistry (IHC) as an aid to the cytological diagnosis of metastatic breast carcinoma in fine needle aspiration (FNA) specimens was investigated.
Cell block sections from 111 FNA cases of metastatic malignancy were stained for GATA3, including metastases from 43 breast and 44 nonmammary adenocarcinomas, 19 melanomas, 4 urothelial carcinomas, and 1 thyroid medullary carcinoma. Sites sampled included lymph nodes (87), bone (8), liver (5), lung (6), superficial masses (4), and pelvic mass (1).
Ninety-one percent (39/43) of metastatic breast carcinoma cases were positive for GATA3. All estrogen receptor (ER)-positive were also GATA3 positive cases. The majority (9/14; 64%) of ER-negative and 37% (3/8) of triple-negative cases were positive for GATA3. All nonmammary adenocarcinoma cases were negative with the exception of one case of metastatic pancreatic adenocarcinoma. Metastatic melanoma cases were all negative but 75% (3/4) urothelial carcinomas expressed GATA3.
GATA3 IHC staining is a useful addition to IHC panels for FNA samples in specific settings such as distinguishing metastatic breast from lung carcinoma or melanoma.
研究GATA3免疫组织化学（IHC）在细针穿刺抽吸（FNA）标本中辅助转移性乳腺癌细胞学诊断的效用。
对111例转移性恶性肿瘤FNA病例的细胞块切片进行GATA3染色，包括43例乳腺癌和44例非乳腺腺癌、19例黑色素瘤、4例尿路上皮癌和1例甲状腺髓样癌的转移灶。取样部位包括淋巴结（87例）、骨（8例）、肝（5例）、肺（6例）、浅表肿物（4例）和盆腔肿物（1例）。
91%（39/43）的转移性乳腺癌病例GATA3呈阳性。所有雌激素受体（ER）阳性病例GATA3也呈阳性。大多数ER阴性病例（9/14；64%）和37%（3/8）的三阴性病例GATA3呈阳性。除1例转移性胰腺腺癌外，所有非乳腺腺癌病例均为阴性。转移性黑色素瘤病例均为阴性，但75%（3/4）的尿路上皮癌表达GATA3。
在特定情况下，如区分转移性乳腺癌与肺癌或黑色素瘤时，GATA3 IHC染色是FNA样本免疫组化检测的有益补充。
