Dept. of Oriental Medicinal Material and Processing, College of Life science, Kyung Hee University, 1 Seocheon, Giheung-gu Yongin-si, Gyeonggi-do 449-701, Republic of Korea.
Plant Physiol Biochem. 2010 Jun;48(6):451-60. doi: 10.1016/j.plaphy.2010.02.005. Epub 2010 Mar 3.
A cDNA clone containing a catalase (CAT1) gene, designated PgCat1, was isolated from Panax ginseng C.A. Meyer (Korean ginseng). PgCat1 is predicted to encode a precursor protein of 492 amino acid residues, and its sequence shares high degrees of homology with a number of other CAT1s. Genomic DNA hybridization analysis indicated that PgCat1 represents a multi-gene family. Reverse transcriptase (RT)-PCR results showed that PgCat1 expressed at different levels in leaves, stem, roots of P. ginseng seedlings. Different stresses, heavy metals, plant hormones, osmotic agents, high light irradiance, abiotic stresses, triggered a significant induction of PgCat1. The positive responses of PgCat1 to the various stimuli suggested that P. ginseng PgCat1 may help to protect the plant against reactive oxidant related environmental stresses.
从 Panax ginseng C.A. Meyer（高丽参）中分离到一个包含过氧化氢酶（CAT1）基因的 cDNA 克隆，命名为 PgCat1。PgCat1 预测编码一个 492 个氨基酸残基的前体蛋白，其序列与许多其他 CAT1 具有高度同源性。基因组 DNA 杂交分析表明，PgCat1 代表一个多基因家族。逆转录酶（RT）-PCR 结果表明，PgCat1 在人参幼苗的叶片、茎、根中表达水平不同。不同的胁迫、重金属、植物激素、渗透剂、高光照射、非生物胁迫都会引发 PgCat1 的显著诱导。PgCat1 对各种刺激的阳性反应表明，人参 PgCat1 可能有助于保护植物免受与活性氧化剂相关的环境胁迫。
