Medical Proteomics Unit, Office for Research and Development, Faculty of Medicine, Siriraj Hospital, and Center for Research in Complex Systems Sciences, Mahidol University, Bangkok 10700, Thailand.
Anal Biochem. 2010 Jul 1;402(1):110-2. doi: 10.1016/j.ab.2010.03.031. Epub 2010 Mar 27.
Artificial urine (AU) is widely used for in vitro cellular study to simulate normal physiological environment of the kidney and urinary tract. However, some compositions of many of previously established AU formulas are out of their physiological ranges in normal human urine. Therefore, we established a new AU formula, named "AU-Siriraj," and then performed systematic comparisons of AU-Siriraj with other six previously established AU formulas, ultrafiltrated (UF) urine, and blank-control to determine their compatibility with MDCK cells. The data indicate that AU-Siriraj is the best, and AU-3 is the second best, AU formula for in vitro cellular study.
人工尿液（AU）广泛用于体外细胞研究，以模拟肾脏和泌尿道的正常生理环境。然而，许多已建立的 AU 配方的一些成分超出了正常人体尿液的生理范围。因此，我们建立了一个新的 AU 配方，命名为“AU-Siriraj”，然后对 AU-Siriraj 与其他六个先前建立的 AU 配方、超滤（UF）尿液和空白对照进行了系统比较，以确定它们与 MDCK 细胞的相容性。数据表明，AU-Siriraj 是体外细胞研究的最佳 AU 配方，AU-3 是第二佳 AU 配方。
