Armutcu Canan, Pişkin Sena, Özgür Erdoğan, Karakaya Mustafa, Çorman M Emin, Uzun Lokman
Faculty of Science, Department of Chemistry, Hacettepe University, Ankara, Turkey.
Faculty of Engineering & Architecture, Department of Energy Systems, Sinop University, Sinop, Turkey.
Mikrochim Acta. 2025 Sep 12;192(10):662. doi: 10.1007/s00604-025-07502-5.
A molecularly imprinted electrochemical sensor (MIP) was developed using thymine-functionalized carbon nanofibers (Thy@CNFs) to enable selective detection of hypoxanthine (HYP). The sensor was fabricated by first depositing Thy@CNFs onto a glassy carbon electrode (GCE), followed by electropolymerization of a pyrrole-co-pyrrole-3-carboxylic acid (Py-co-PyCOOH) copolymer in the presence of HYP. Each modification step was characterized using electrochemical impedance spectroscopy (EIS), cyclic voltammetry (CV), attenuated total reflection-Fourier transform infrared spectroscopy (ATR-FTIR), scanning electron microscopy (SEM), and contact angle measurements. Under optimized conditions, the Thy@CNFs-modified MIP sensor (Thy@CNFs/MIP/GCE) exhibited a linear response to HYP concentrations ranging from 1 × 10 to 1 × 10 M, with a detection limit of 1.71 × 10 M. Finally, the sensor was successfully applied to commercial serum and artificial urine sample, achieving recoveries of 99.55% and 100.17%, respectively, demonstrating its accuracy, precision, and practical applicability in real sample analysis.
利用胸腺嘧啶功能化的碳纳米纤维（Thy@CNFs）开发了一种分子印迹电化学传感器（MIP），用于选择性检测次黄嘌呤（HYP）。该传感器的制备方法是先将Thy@CNFs沉积在玻碳电极（GCE）上，然后在HYP存在的情况下对吡咯 - 共 - 吡咯 - 3 - 羧酸（Py - co - PyCOOH）共聚物进行电聚合。每个修饰步骤都使用电化学阻抗谱（EIS）、循环伏安法（CV）、衰减全反射 - 傅里叶变换红外光谱（ATR - FTIR）、扫描电子显微镜（SEM）和接触角测量进行表征。在优化条件下，Thy@CNFs修饰的MIP传感器（Thy@CNFs/MIP/GCE）对浓度范围为1×10至1×10 M的HYP表现出线性响应，检测限为1.71×10 M。最后，该传感器成功应用于商业血清和人工尿液样本，回收率分别为99.55%和100.17%，证明了其在实际样品分析中的准确性、精密度和实际适用性。
