Department of Biochemistry and Physiology of Plants, Faculty of Biology, University of Bielefeld, D-33501 Bielefeld, Germany.
Anal Biochem. 2010 Jul 1;402(1):105-6. doi: 10.1016/j.ab.2010.03.026. Epub 2010 Mar 27.
We present, for the red fluorescent protein mCherry acting as both fluorescence resonant energy transfer (FRET) donor and acceptor, Förster critical distance (r(0)) values with five important visible fluorescent protein (VFP) variants as well as with itself. The pair EYFP-mCherry exhibits an r(0) of 5.66nm, equaling or exceeding any combination of VFPs reported previously. Moreover, mCherry should be an excellent chromophore for homo-FRET with an r(0) of 5.10nm for energy transfer between two mCherry moieties. Finally, mCherry exhibits higher r(0) values than does DsRed. These characteristics, combined with mCherry's rapid folding and excellent spectral properties, suggest that mCherry constitutes a valuable long-wavelength hetero-FRET acceptor and probe for homo-FRET experiments.
我们提出，对于既作为荧光共振能量转移（FRET）供体又作为受体的红色荧光蛋白 mCherry，与五个重要的可见荧光蛋白（VFP）变体以及自身相比，福斯特临界距离（r(0)）值。EYFP-mCherry 对显示出 5.66nm 的 r(0)，等于或超过以前报道的任何 VFP 组合。此外，mCherry 应该是同型 FRET 的极好发色团，两个 mCherry 部分之间的能量转移 r(0)为 5.10nm。最后，mCherry 的 r(0)值高于 DsRed。这些特性，加上 mCherry 快速折叠和出色的光谱特性，表明 mCherry 构成了一种有价值的长波长异 FRET 受体和同型 FRET 实验的探针。
