Biotechnology Center, 2 St John's Close, University of the West Indies, Mona Campus, Kingston, Jamaica.
Virus Res. 2010 Jun;150(1-2):148-52. doi: 10.1016/j.virusres.2010.03.008. Epub 2010 Mar 25.
Partial genome sequences for the tentative begomovirus Macroptilium golden mosaic virus (MGMV) have been previously reported and were originally obtained for an isolate that infected Macroptilium lathyroides in Jamaica. In this study, we PCR-amplified, cloned and determined the sequence for the complete genome of isolates of MGMV that we found infecting Wissadula amplissima collected from August Town and Spanish Town, Jamaica. Sequence analysis confirmed that MGMV is a distinct begomovirus species, based on the ICTV 89% rule for species demarcation. MGMV shared its highest nucleotide identity at 79% for DNA-A component and 66% for DNA-B component to Corchorus yellow spot virus [Mexico:Yucatan:2005]. The names Macroptilium golden mosaic virus [Jamaica1:Wissadula:AugustTown] (MGMV [JM1:Wd:AT]) and Macroptilium golden mosaic virus [Jamaica1:Wissadula:SpanishTown] (MGMV [JM1:Wd:ST]) are proposed herein for the MGMV isolates from August Town and Spanish Town, respectively. The genome organization of MGMV [JM1:Wd:AT] and MGMV [JM1:Wd:ST] is characteristic of Western Hemisphere bipartite begomoviruses. Excluding the replication enhancer protein (REn), all proteins encoded by the MGMV [JM1:Wd:AT] and MGMV [JM1:Wd:ST] genomes are most similar to their counterparts in Western Hemisphere begomoviruses. The REn proteins of MGMV [JM1:Wd:AT] and MGMV [JM1:Wd:ST], share greatest similarity to the REn protein of Corchorus yellow vein virus [Vietnam:Hoa Binh:2000], a New World-like begomovirus identified in Asia. Phylogenetic reconstruction places MGMV in a clade containing Potato yellow mosaic virus. Results of an experimental host range study indicated that MGMV [JM1:Wd:AT] can infect kidney bean, hot pepper and tomato.
先前已经报道了暂定的豆薯金色花叶病毒（MGMV）的部分基因组序列，这些序列最初是从感染牙买加Macroptilium lathyroides 的分离株中获得的。在这项研究中，我们通过 PCR 扩增、克隆并确定了我们在牙买加 August Town 和 Spanish Town 采集的 Wissadula amplissima 感染的 MGMV 分离株的全基因组序列。序列分析证实，根据 ICTV 89%的物种划分规则，MGMV 是一种独特的黄症病毒物种。MGMV 在 DNA-A 组分上的核苷酸同一性最高为 79%，在 DNA-B 组分上的核苷酸同一性最高为 66%，与 Corchorus yellow spot virus [墨西哥：尤卡坦：2005] 同源。本研究提出了来自 August Town 和 Spanish Town 的 MGMV 分离株的名称，Macroptilium golden mosaic virus [牙买加 1：Wissadula：August Town]（MGMV [JM1：Wd：AT]）和 Macroptilium golden mosaic virus [牙买加 1：Wissadula：Spanish Town]（MGMV [JM1：Wd：ST]）。MGMV [JM1：Wd：AT] 和 MGMV [JM1：Wd：ST] 的基因组组织特征是西半球二分体伴生病毒。排除复制增强蛋白（REn），MGMV [JM1：Wd：AT] 和 MGMV [JM1：Wd：ST] 基因组编码的所有蛋白与西半球伴生病毒的对应蛋白最为相似。MGMV [JM1：Wd：AT] 和 MGMV [JM1：Wd：ST] 的 REn 蛋白与亚洲鉴定的新型伴生病毒 Corchorus yellow vein virus [越南：和平：2000] 的 REn 蛋白最相似。系统发育重建将 MGMV 置于一个包含马铃薯黄斑花叶病毒的分支中。一项实验宿主范围研究的结果表明，MGMV [JM1：Wd：AT] 可以感染菜豆、辣椒和番茄。
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