Biotechnology Centre, 2 St. John's Close, University of the West Indies, Mona, Kingston 7, Jamaica,
Arch Virol. 2014 Feb;159(2):375-8. doi: 10.1007/s00705-013-1814-4. Epub 2013 Aug 11.
The complete DNA sequence of both genome components of a new begomovirus (Sida golden mosaic Buckup virus-[Jamaica:St. Elizabeth:2004]; SiGMBuV-[JM:SE:04]) was determined from a field-infected Sida sp. sample from Buckup, St. Elizabeth, Jamaica. Phylogenetically, both genome components of SiGMBuV-[JM:SE:04] are most closely related to malvaceous weed-infecting Floridian and Mexican begomoviruses. Its DNA-B is a recombinant molecule, the majority of which was derived from a virus resembling Sida yellow mosaic Yucatan virus-[Mexico:Yucatan:2005] (SiYMYuV-[MX:Yuc:05]), while nucleotides 43-342 were derived from a virus resembling Sida golden mosaic virus-[United States of America:Florida] (SiGMV-[US:Flo]). Symptomatic infectivity of our cloned SiGMBuV-[JM:SE:04] components was confirmed in Nicotiana benthamiana.
新的黄脉病毒(西达黄花叶病毒- [牙买加:圣伊丽莎白:2004];SiGMBuV-[JM:SE:04])的两个基因组成分的完整 DNA 序列是从牙买加 Buckup 的田间受感染的 Sida sp.样本中确定的。系统发育分析表明,SiGMBuV-[JM:SE:04]的两个基因组成分与感染草科杂草的佛罗里达和墨西哥黄脉病毒最为密切相关。其 DNA-B 是一个重组分子,主要来源于与 Sida 黄花叶病毒-尤卡坦病毒-[墨西哥:尤卡坦:2005](SiYMYuV-[MX:Yuc:05])相似的病毒,而核苷酸 43-342 来源于与 Sida 黄花叶病毒-美国佛罗里达州病毒相似的病毒(SiGMV-[US:Flo])。我们克隆的 SiGMBuV-[JM:SE:04] 成分在本氏烟中证实了有症状的感染性。