首次报道来自牙买加的 Sida golden yellow vein 病毒的完整序列。
First report of the complete sequence of Sida golden yellow vein virus from Jamaica.
机构信息
The Biotechnology Centre, The University of the West Indies, 2 St. John's Close, Mona, Jamaica.
出版信息
Arch Virol. 2011 Aug;156(8):1481-4. doi: 10.1007/s00705-011-1030-z. Epub 2011 May 29.
Begomoviruses are phytopathogens that threaten food security [18]. Sida spp. are ubiquitous weed species found in Jamaica. Sida samples were collected island-wide, DNA was extracted via a modified Dellaporta method, and the viral genome was amplified using degenerate and sequence-specific primers [2, 11]. The amplicons were cloned and sequenced. Sequence analysis revealed that a DNA-A molecule isolated from a plant in Liguanea, St. Andrew, was 90.9% similar to Sida golden yellow vein virus-[United States of America:Homestead:A11], making it a strain of SiGYVV. It was named Sida golden yellow vein virus-[Jamaica:Liguanea 2:2008] (SiGYVV-[JM:Lig2:08]). The cognate DNA-B, previously unreported, was successfully cloned and was most similar to that of Malvastrum yellow mosaic Jamaica virus (MaYMJV). Phylogenetic analysis suggested that this virus was most closely related to begomoviruses that infect malvaceous hosts in Jamaica, Cuba and Florida in the United States.
双生病毒是威胁粮食安全的植物病原体[18]。赛葵属是在牙买加普遍存在的杂草物种。在全岛范围内采集赛葵样本，通过改良的 Dellaporta 方法提取 DNA，并使用简并和序列特异性引物[2,11]扩增病毒基因组。扩增子被克隆和测序。序列分析表明，从圣安德鲁的利瓜内亚一株植物中分离出的 DNA-A 分子与 Sida 金黄色叶脉病毒-[美国：霍姆斯特德：A11]的相似度为 90.9%，使其成为 SiGYVV 的一个株系。它被命名为 Sida 金黄色叶脉病毒-[牙买加：利瓜内亚 2：2008]（SiGYVV-[JM:Lig2:08]）。以前未报道过的相应 DNA-B 成功克隆，与马拉维黄脉花叶病毒（MaYMJV）最为相似。系统发育分析表明，该病毒与感染牙买加、古巴和美国佛罗里达州的锦葵科植物的双生病毒最为密切相关。
Zotero 插件