尼瓦诺卡氏菌（Nocardia niwae），从人源肺部分离得到。
Nocardia niwae sp. nov., isolated from human pulmonary sources.
机构信息
Bacterial Zoonoses Branch, Division of Foodborne, Bacterial and Mycotic Diseases, National Center for Zoonotic, Vector-borne and Enteric Diseases, Centers for Disease Control and Prevention, Atlanta, GA 30333, USA.
German Collection of Microorganisms and Cell Cultures, Braunschweig, Germany.
出版信息
Int J Syst Evol Microbiol. 2011 Feb;61(Pt 2):438-442. doi: 10.1099/ijs.0.020370-0. Epub 2010 Mar 26.
Members of the genus Nocardia are responsible for cutaneous, pulmonary and disseminated human infections. From 2003 to 2008, four nocardioform strains (W8027, W8681, W9071 and W9241(T)) were isolated from patients in the state of Florida, USA. Ribosomal gene sequencing analysis suggested that a novel species of the genus Nocardia had been isolated. These strains were subjected to a taxonomic analysis using a polyphasic approach. Phenotypic analyses included morphological examination, biochemical profiling and antimicrobial susceptibility testing. Molecular studies included 16S rRNA and DNA gyrase B subunit (gyrB) gene sequence analyses and DNA-DNA hybridization. Phylogenetic neighbours were determined through 16S rRNA and gyrB gene sequence analyses. Phenotypic characteristics that differentiated the novel isolates from phylogenetically related species were growth at 45 °C, and three of the four novel strains utilized l-rhamnose. The antimicrobial profiles could not reliably distinguish the novel species from related nocardiae. Analysis showed that the 16S rRNA gene sequences of the four novel isolates were identical. The blast analysis of the near full-length 16S rRNA gene showed 99.2 % sequence similarity to Nocardia araoensis DSM 44729(T), Nocardia arthritidis DSM 44731(T) and Nocardia beijingensis JCM 10666(T), 98.7 % to Nocardia amamiensis DSM 45066(T), 98.2 % to Nocardia pneumoniae JCM 12119(T) and 97.8 % to Nocardia takedensis JCM 13313(T). Analysis of partial gyrB gene sequences showed that the novel isolates had 95.4 % similarity to N. arthritidis DSM 44731(T), 95.3 % to Nocardia gamkensis DSM 44956(T), 94.4 % to N. pneumoniae JCM 12119(T), 93.8 % to Nocardia asiatica DSM 44668(T), 93.5 % to N. amamiensis DSM 45066(T), 93.4 % to N. beijingensis JCM 10666(T) and 93.2 % to N. araoensis DSM 44729(T). The DNA-DNA relatedness values between the four novel strains were 86-89 %; the relatedness value for strain W9241(T) compared with N. beijingensis JCM 10666(T) was 47 % and 46 % with N. araoensis DSM 44729(T), 44 % with N. arthritidis DSM 44731(T), 32 % with N. amamiensis DSM 45066(T) and 20 % with N. asiatica DSM 44668(T). The results of the taxonomic analysis suggested that the new isolates represent a novel species of the genus Nocardia for which the name Nocardia niwae sp. nov. is proposed. The type strain is W9241(T) (=DSM 45340(T)=CCUG 57756(T)).
从 2003 年到 2008 年，从美国佛罗里达州的患者中分离出了 4 株诺卡氏菌（W8027、W8681、W9071 和 W9241(T)）。核糖体基因测序分析表明，一种新型的诺卡氏菌已被分离出来。这些菌株采用多相方法进行了分类分析。表型分析包括形态学检查、生化特征分析和抗菌药物敏感性试验。分子研究包括 16S rRNA 和 DNA 拓扑异构酶 B 亚基（gyrB）基因序列分析和 DNA-DNA 杂交。通过 16S rRNA 和 gyrB 基因序列分析确定了系统发育上的近缘种。新型分离株与系统发育上相关的种区分的表型特征是在 45°C 下生长，以及其中的四个新型菌株利用 l-鼠李糖。抗菌药物谱无法可靠地区分新型种与相关的诺卡氏菌。分析表明，四个新型分离株的 16S rRNA 基因序列完全相同。近全长 16S rRNA 基因的 blast 分析显示，与 Nocardia araoensis DSM 44729(T)、Nocardia arthritidis DSM 44731(T)和 Nocardia beijingensis JCM 10666(T)的序列相似性为 99.2%，与 Nocardia amamiensis DSM 45066(T)的序列相似性为 98.7%，与 Nocardia pneumoniae JCM 12119(T)的序列相似性为 98.2%，与 Nocardia takedensis JCM 13313(T)的序列相似性为 97.8%。gyrB 基因部分序列分析表明，新型分离株与 Nocardia arthritidis DSM 44731(T)的相似度为 95.4%，与 Nocardia gamkensis DSM 44956(T)的相似度为 95.3%，与 Nocardia pneumoniae JCM 12119(T)的相似度为 94.4%，与 Nocardia asiatica DSM 44668(T)的相似度为 93.8%，与 Nocardia amamiensis DSM 45066(T)的相似度为 93.5%，与 Nocardia beijingensis JCM 10666(T)的相似度为 93.4%，与 Nocardia araoensis DSM 44729(T)的相似度为 93.2%。四个新型菌株之间的 DNA-DNA 相关值为 86-89%；与 Nocardia beijingensis JCM 10666(T)相比，菌株 W9241(T)的相关值为 47%和 46%与 Nocardia araoensis DSM 44729(T)，44%与 Nocardia arthritidis DSM 44731(T)，32%与 Nocardia amamiensis DSM 45066(T)和 20%与 Nocardia asiatica DSM 44668(T)。分类分析结果表明，新分离株代表了一个新型的诺卡氏菌属，建议将其命名为 Nocardia niwae sp. nov.。模式菌株为 W9241(T)（=DSM 45340(T)=CCUG 57756(T)）。
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