Yamamura Hideki, Tamura Tomohiko, Sakiyama Yayoi, Harayama Shigeaki
NITE Biological Resource Center, National Institute of Technology and Evaluation, Kazusakamatari 2-5-8, Kisarazu, Chiba 292-0818, Japan.
Int J Syst Evol Microbiol. 2007 Jul;57(Pt 7):1599-1602. doi: 10.1099/ijs.0.64829-0.
An actinomycete, strain TT 00-78(T), was isolated from soil from a sugar-cane field on Amami Island in Japan, using an SDS/yeast extract pre-treatment method, and the taxonomy was studied using a polyphasic approach. The chemotaxonomic and morphological characterizations clearly demonstrated that the strain belongs to the genus Nocardia. 16S rRNA gene sequencing studies showed that the strain was closely related to the type strains of Nocardia pneumoniae (98.6 %), Nocardia araoensis (98.1 %), Nocardia arthritidis (97.9 %) and Nocardia beijingensis (97.7 %). However, the results of DNA-DNA hybridization and physiological and biochemical tests showed that strain TT 00-78(T) could be differentiated from its closest phylogenetic relatives both genotypically and phenotypically. Therefore this strain represents a novel species of the genus Nocardia, for which the name Nocardia amamiensis sp. nov. is proposed. The type strain is TT 00-78(T) (=NBRC 102102(T)=DSM 45066(T)=KCTC 19208(T)).
从日本奄美大岛甘蔗田土壤中，采用SDS/酵母提取物预处理方法分离出一株放线菌，菌株编号为TT 00-78(T)，并采用多相分类方法对其进行分类学研究。化学分类学和形态学特征明确表明，该菌株属于诺卡氏菌属。16S rRNA基因测序研究表明，该菌株与肺炎诺卡氏菌（98.6%）、荒川诺卡氏菌（98.1%）、关节炎诺卡氏菌（97.9%）和北京诺卡氏菌（97.7%）的模式菌株密切相关。然而，DNA-DNA杂交以及生理生化试验结果表明，菌株TT 00-78(T)在基因型和表型上均与其最相近的系统发育亲缘种有所不同。因此，该菌株代表诺卡氏菌属的一个新物种，为此提出新名称诺卡氏菌奄美亚种（Nocardia amamiensis sp. nov.）。模式菌株为TT 00-78(T)（=NBRC 102102(T)=DSM 45066(T)=KCTC 19208(T)）。
