实时聚合酶链反应评估菲律宾水牛中日本血吸虫感染的高流行率。
High prevalence of Schistosoma japonicum infection in water buffaloes in the Philippines assessed by real-time polymerase chain reaction.
机构信息
Department of Pathogen Biology, Nanjing Medical University, Nanjing, China.
出版信息
Am J Trop Med Hyg. 2010 Apr;82(4):646-52. doi: 10.4269/ajtmh.2010.09-0638.
Difficulty in controlling human Schistosoma japonicum infection is partly attributed to the presence of non-human definitive hosts. Water buffaloes are a major reservoir for transmission of S. japonicum to humans in China. However, in the Philippines, reports based on microscopic examination of buffalo stool identified a low prevalence of S. japonicum, and mathematical models using these data concluded that water buffaloes are not a major reservoir for transmission of S. japonicum to humans. We collected stool from 81 buffaloes in Macanip, Leyte, the Philippines, and assayed for S. japonicum infection by the Danish Bilharziasis Laboratory technique, the Kato-Katz technique, miracidia hatching, and a highly validated real-time polymerase chain reaction. The prevalence defined by each assay was 3.7%, 3.7%, 0%, and 51.5% respectively. Our results demonstrate that microscopic-based techniques dramatically underestimate the prevalence of S. japonicum infection in water buffaloes in the Philippines and warrant reexamination of the role of bovines in transmission of S. japonicum to humans in the Philippines.
人类感染日本血吸虫的控制困难部分归因于非人类的终宿主的存在。水牛是中国日本血吸虫传播给人类的主要储存宿主。然而，在菲律宾，基于对水牛粪便进行显微镜检查的报告表明，日本血吸虫的流行率较低，并且使用这些数据的数学模型得出的结论是，水牛不是日本血吸虫传播给人类的主要储存宿主。我们从菲律宾莱特省马卡尼普收集了 81 头水牛的粪便，并通过丹麦血吸虫病实验室技术、加藤加藤技术、尾蚴孵化和高度验证的实时聚合酶链反应检测日本血吸虫感染。每种检测方法定义的流行率分别为 3.7%、3.7%、0%和 51.5%。我们的结果表明，基于显微镜的技术大大低估了菲律宾水牛感染日本血吸虫的流行率，有必要重新审查牛在菲律宾日本血吸虫向人类传播中的作用。
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