Lefkowitz R J
Fed Proc. 1978 Feb;37(2):123-9.
Direct radioligand binding methods for studying the alpha- and beta-adrenergic receptors have been developed over the past several years. These techniques use radioactively labeled adrenergic antagonists and agonists to identify the receptors in appropriate membrane fractions from catecholamine-sensitive tissues. In the case of the beta-adrenergic receptors, confident receptor identification has been aided by the close correlation of binding data with data on adenylate cyclase activation. Such direct binding studies are providing new insights about the molecular characteristics and regulatory properties of the receptors.
在过去几年中，已经开发出用于研究α和β肾上腺素能受体的直接放射性配体结合方法。这些技术使用放射性标记的肾上腺素能拮抗剂和激动剂来鉴定来自儿茶酚胺敏感组织的合适膜组分中的受体。就β肾上腺素能受体而言，结合数据与腺苷酸环化酶激活数据的密切相关性有助于可靠地鉴定受体。此类直接结合研究正在为受体的分子特征和调节特性提供新的见解。
