Strosberg A D, Couraud P O, Delavier-Klutchko C, Durieu-Trautmann O, Schmutz A, Lu B Z
Ann Immunol (Paris). 1982 Sep-Oct;133D(2):191-7.
Two types of antibodies have been prepared against beta-adrenergic receptors: (1) antibodies against receptor, purified from turkey erythrocyte membranes by affinity chromatography on alprenolol sepharose, and (2) antiidiotypic antibodies raised against the anti-alprenolol immunoglobulins. Both types of antibodies specifically bind to cells which possess beta-adrenergic receptor and mimick the biological effect of the catecholamine hormone: they stimulate basal adenylate-cyclase and enhance adenylate-cyclase activation by catecholamines. The antiidiotypic antibodies only compete with (--)-3H-dihydroalprenolol for binding to the beta-adrenergic receptors on purified turkey erythrocyte membranes. The use of these antibodies as tools for the study of the mechanism of the beta-adrenergic receptor-cyclase complex is discussed.
已制备出两种针对β-肾上腺素能受体的抗体：（1）通过在烯丙洛尔琼脂糖上进行亲和层析从火鸡红细胞膜中纯化得到的抗受体抗体，以及（2）针对抗烯丙洛尔免疫球蛋白产生的抗独特型抗体。这两种抗体都能特异性地与具有β-肾上腺素能受体的细胞结合，并模拟儿茶酚胺激素的生物学效应：它们刺激基础腺苷酸环化酶，并增强儿茶酚胺对腺苷酸环化酶的激活作用。抗独特型抗体仅与（-）-3H-二氢烯丙洛尔竞争结合纯化的火鸡红细胞膜上的β-肾上腺素能受体。本文讨论了将这些抗体用作研究β-肾上腺素能受体-环化酶复合物机制的工具。
